ArticleJournal of lipid research2012
Quantifying apoprotein synthesis in rodents: coupling LC-MS/MS analyses with the administration of labeled water.
Article in Journal of lipid research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Directly Measuring Atherogenic Lipoprotein Kinetics in Zebrafish With the Photoconvertible LipoTimer Reporter.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- Measurement of lipid flux to advance translational research: evolution of classic methods to the future of precision health.Experimental & molecular medicine · 2022Review
- Glucagon receptor antagonism induces increased cholesterol absorption.Journal of lipid research · 2015Article
- Tracer-based estimates of protein flux in cases of incomplete product renewal: evidence and implications of heterogeneity in collagen turnover.American journal of physiology. Endocrinology and metabolism · 2015Article
- Quantifying protein synthesis and degradation in Arabidopsis by dynamic 13CO2 labeling and analysis of enrichment in individual amino acids in their free pools and in protein.Plant physiology · 2015Article
- Ceramide as a mediator of non-alcoholic Fatty liver disease and associated atherosclerosis.PloS one · 2015Article
- Practical immunoaffinity-enrichment LC-MS for measuring protein kinetics of low-abundance proteins.Clinical chemistry · 2014Article
- 2H2O-based high-density lipoprotein turnover method for the assessment of dynamic high-density lipoprotein function in mice.Arteriosclerosis, thrombosis, and vascular biology · 2013Article
- Measurement of apo(a) kinetics in human subjects using a microfluidic device with tandem mass spectrometry.Rapid communications in mass spectrometry : RCM · 2013Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable isotope tracer studies of apoprotein flux in rodent models present difficulties as they require working with small volumes of plasma. We demonstrate the ability to measure apoprotein flux by administering either (2)H- or (18)O-labeled water to mice and then subjecting samples to LC-MS/MS analyses; we were able to simultaneously determine the labeling of several proteolytic peptides representing multiple apoproteins. Consistent with relative differences reported in the literature regarding apoprotein flux in humans, we found that the fractional synthetic rate of apoB is greater than apoA1 in mice. In addition, the method is suitable for quantifying acute changes in protein flux: we observed a stimulation of apoB production in mice following an intravenous injection of Intralipid and a decrease in apoB production in mice treated with an inhibitor of microsomal triglyceride transfer protein. In summary, we demonstrate a high-throughput method for studying apoprotein kinetics in rodent models. Although notable differences exist between lipoprotein profiles that are observed in rodents and humans, we expect that the method reported here has merit in studies of dyslipidemia as i) rodent models can be used to probe target engagement in cases where one aims to modulate apoprotein production and ii) the approach should be adaptable to studies in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.