ArticlePlant physiology2012
Loss of Cellulose synthase-like F6 function affects mixed-linkage glucan deposition, cell wall mechanical properties, and defense responses in vegetative tissues of rice.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
93 citing papers in PubMed, 1 synthesis or guideline pooled it, 183 citations in OpenAlex.
- Meta-analysis of drought-tolerant genotypes in Oryza sativa: A network-based approach.PloS one · 2019Pooled it
- Article
- The Plant Cell Wall: From Structural Diversity and Biosynthesis to Integrity Signaling and Physiological Functions.Plants (Basel, Switzerland) · 2026Review
- Integrating molecular networks and physiological adaptation for heat-resilient crops.Journal of integrative plant biology · 2026Review
- Genetic Interaction Between Bph50 and Bph51 Confers Resistance to Brown Planthopper in Rice.Rice (New York, N.Y.) · 2026Article
- Integrative molecular mechanisms of lodging resistance in rice (Frontiers in plant science · 2026Review
- Decreased Expression of a Phosphoribosylanthranilate Transferase-Encoding Gene,International journal of molecular sciences · 2025Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- OsCDPK24 and OsCDPK28 phosphorylate heat shock factor OsHSFA4d to orchestrate abiotic and biotic stress responses in rice.Nature communications · 2025Article
- Integrated Metabolomics and Proteomics Analyses of the Grain-Filling Process and Differences in the Quality of Tibetan Hulless Barleys.Plants (Basel, Switzerland) · 2025Article
- The Overexpression ofPlants (Basel, Switzerland) · 2025Article
- Lesion Mimic Mutant: An Ideal Genetic Material for Deciphering the Balance Between Plant Immunity and Growth.Rice (New York, N.Y.) · 2025Review
- Comparative transcriptome analysis reveals potential regulatory genes involved in the development and strength formation of maize stalks.BMC plant biology · 2025Article
- Genome-wide identification and expression analysis of theFrontiers in plant science · 2025Article
- Is theFood chemistry. Molecular sciences · 2024Article
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2024Article
- Potential Roles of the GRF Transcription Factors in Sorghum Internodes during Post-Reproductive Stages.Plants (Basel, Switzerland) · 2024Article
- Research Progress on Mechanical Strength of Rice Stalks.Plants (Basel, Switzerland) · 2024Review
- Transcriptomic analysis of the response ofFrontiers in microbiology · 2024Article
- Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mixed-linkage glucan (MLG) is a cell wall polysaccharide containing a backbone of unbranched (1,3)- and (1,4)-linked β-glucosyl residues. Based on its occurrence in plants and chemical characteristics, MLG has primarily been associated with the regulation of cell wall expansion due to its high and transient accumulation in young, expanding tissues. The Cellulose synthase-like F (CslF) subfamily of glycosyltransferases has previously been implicated in mediating the biosynthesis of this polymer. We confirmed that the rice (Oryza sativa) CslF6 gene mediates the biosynthesis of MLG by overexpressing it in Nicotiana benthamiana. Rice cslf6 knockout mutants show a slight decrease in height and stem diameter but otherwise grew normally during vegetative development. However, cslf6 mutants display a drastic decrease in MLG content (97% reduction in coleoptiles and virtually undetectable in other tissues). Immunodetection with an anti-MLG monoclonal antibody revealed that the coleoptiles and leaves retain trace amounts of MLG only in specific cell types such as sclerenchyma fibers. These results correlate with the absence of endogenous MLG synthase activity in mutant seedlings and 4-week-old sheaths. Mutant cell walls are weaker in mature stems but not seedlings, and more brittle in both stems and seedlings, compared to wild type. Mutants also display lesion mimic phenotypes in leaves, which correlates with enhanced defense-related gene expression and enhanced disease resistance. Taken together, our results underline a weaker role of MLG in cell expansion than previously thought, and highlight a structural role for MLG in nonexpanding, mature stem tissues in rice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.