ArticleAnalytical biochemistry2012
Imaging lysosomal enzyme activity in live cells using self-quenched substrates.
Article in Analytical biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- A toxic gain-of-function mechanism in C9orf72 ALS impairs the autophagy-lysosome pathway in neurons.Acta neuropathologica communications · 2023Article
- Microglial phagocytosis dysfunction in stroke is driven by energy depletion and induction of autophagy.Autophagy · 2023Article
- Article
- Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway.The Journal of cell biology · 2022Article
- Repurposing Itraconazole and Hydroxychloroquine to Target Lysosomal Homeostasis in Epithelial Ovarian Cancer.Cancer research communications · 2022Article
- Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis.Biophysical journal · 2022Article
- ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.Developmental cell · 2021Article
- Article
- Article
- Liver sinusoidal endothelial cells contribute to the uptake and degradation of entero bacterial viruses.Scientific reports · 2020Article
- Bacterial LPX motif-harboring virulence factors constitute a species-spanning family of cell-penetrating effectors.Cellular and molecular life sciences : CMLS · 2018Article
- T3SS-Independent Uptake of the Short-Trip Toxin-Related Recombinant NleC Effector of EnteropathogenicFrontiers in cellular and infection microbiology · 2017Article
- Improved analysis for determining diffusion coefficients from short, single-molecule trajectories with photoblinking.Langmuir : the ACS journal of surfaces and colloids · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Endocytosis, the internalization and transport of extracellular cargo, is an essential cellular process. The ultimate step in endocytosis is the intracellular degradation of extracellular cargo for use by the cell. While live cell imaging and single particle tracking have been well-utilized to study the internalization and transport of cargo, the final degradation step has required separate biochemical assays. We describe the use of self-quenched endocytic cargo to image the intracellular transport and degradation of endocytic cargo directly in live cells. We first outline the fluorescent labeling and quantification of two common endocytic cargos: a protein, bovine serum albumin, and a lipid nanoparticle, low-density lipoprotein. In vitro measurements confirm that self-quenching is a function of the number of fluorophores bound to the protein or particle and that recovery of the fluorescent signal occurs in response to enzymatic degradation. We then use confocal fluorescence microscopy and flow cytometry to demonstrate the use of self-quenched bovine serum albumin with standard fluorescence techniques. Using live cell imaging and single particle tracking, we find that the degradation of bovine serum albumin occurs in an endo-lysosomal vesicle that is positive for LAMP1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.