ArticleBiophysical journal2012
Imaging protein structure in water at 2.7 nm resolution by transmission electron microscopy.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 109 citations in OpenAlex.
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- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- Electron videography of a lipid-protein tango.Science advances · 2024Article
- Ouzo Effect Examined at the Nanoscale via Direct Observation of Droplet Nucleation and Morphology.ACS central science · 2023Article
- Microfluidics for multiscale studies of biomolecular condensates.Lab on a chip · 2022Review
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- Liquid electron microscopy: then, now and future.Applied microscopy · 2019Review
- Correlative electron and fluorescence microscopy of magnetotactic bacteria in liquid: toward in vivo imaging.Scientific reports · 2014Article
- Dynamic autofluorescence imaging of intracellular components inside living cells using direct electron beam excitation.Biomedical optics express · 2014Article
- Enabling direct nanoscale observations of biological reactions with dynamic TEM.Microscopy (Oxford, England) · 2013Review
- Revealing dynamic processes of materials in liquids using liquid cell transmission electron microscopy.Journal of visualized experiments : JoVE · 2012Article
- Direct in situ determination of the mechanisms controlling nanoparticle nucleation and growth.ACS nano · 2012Article
- Electron microscopy of biological specimens in liquid water.Biophysical journal · 2012Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate an in situ transmission electron microscopy technique for imaging proteins in liquid water at room temperature. Liquid samples are loaded into a microfabricated environmental cell that isolates the sample from the vacuum with thin silicon nitride windows. We show that electron micrographs of acrosomal bundles in water are similar to bundles imaged in ice, and we determined the resolution to be at least 2.7 nm at doses of ∼35 e/Å(2). The resolution was limited by the thickness of the window and radiation damage. Surprisingly, we observed a smaller fall-off in the intensity of reflections in room-temperature water than in 98 K ice. Thus, our technique extends imaging of unstained and unlabeled macromolecular assemblies in water from the resolution of the light microscope to the nanometer resolution of the electron microscope. Our results suggest that real-time imaging of protein dynamics is conceptually feasible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.