ArticleBiophysical journal2012
Using DNA as a fiducial marker to study SMC complex interactions with the atomic force microscope.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Cancer-associated mutations in the condensin II subunit CAPH2 cause genomic instability through telomere dysfunction and anaphase chromosome bridges.Journal of cellular physiology · 2021Article
- Bridging-induced phase separation induced by cohesin SMC protein complexes.Science advances · 2021Article
- The condensin holocomplex cycles dynamically between open and collapsed states.Nature structural & molecular biology · 2020Article
- Bending and looping of long DNA by Polycomb repressive complex 2 revealed by AFM imaging in liquid.Nucleic acids research · 2020Article
- Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.Molecular cell · 2019Article
- Condensins and cohesins - one of these things is not like the other!Journal of cell science · 2019Review
- Article
- The TubR-centromere complex adopts a double-ring segrosome structure in Type III partition systems.Nucleic acids research · 2018Article
- Oligomerization and ATP stimulate condensin-mediated DNA compaction.Scientific reports · 2017Article
- The role of ATP-dependent machines in regulating genome topology.Current opinion in structural biology · 2016Review
- Multistep assembly of DNA condensation clusters by SMC.Nature communications · 2016Article
- SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis.eLife · 2015Article
- Molecular basis for SMC rod formation and its dissolution upon DNA binding.Molecular cell · 2015Article
- The bacterial chromosome: architecture and action of bacterial SMC and SMC-like complexes.FEMS microbiology reviews · 2014Review
- Single molecule analysis of DNA wrapping and looping by a circular 14mer wheel of the bacteriophage 186 CI repressor.Nucleic acids research · 2013Article
- An asymmetric SMC-kleisin bridge in prokaryotic condensin.Nature structural & molecular biology · 2013Article
- Breaking symmetry in SMCs.Nature structural & molecular biology · 2013Article
- Oligomerization and DNA binding of Ler, a master regulator of pathogenicity of enterohemorrhagic and enteropathogenic Escherichia coli.Nucleic acids research · 2012Article
- Condensins: universal organizers of chromosomes with diverse functions.Genes & development · 2012Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Atomic force microscopy can potentially provide information on protein volumes, shapes, and interactions but is susceptible to variable tip-induced artifacts. In this study, we present an atomic force microscopy approach that can measure volumes of nonglobular polypeptides such as structural maintenance of chromosomes (SMC) proteins, and use it to study the interactions that occur within and between SMC complexes. Together with the protein of interest, we coadsorb a DNA molecule and use it as a fiducial marker to account for tip-induced artifacts that affect both protein and DNA, allowing normalization of protein volumes from images taken on different days and with different tips. This approach significantly reduced the error associated with volume analysis, and allowed determination of the oligomeric states and architecture of the Bacillus subtilis SMC complex, formed by the SMC protein, and by the smaller ScpA and ScpB subunits. This work reveals that SMC and ScpB are dimers and that ScpA is a stable monomer. Moreover, whereas ScpA binds directly to SMC, ScpB only binds to SMC in the presence of ScpA. Notably, the presence of both ScpA and ScpB favored the formation of higher-order structures of SMC complexes, suggesting a role for these subunits in the organization of SMC oligomers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.