ArticleBiophysical journal2012
Single-molecule analysis of the microtubule cross-linking protein MAP65-1 reveals a molecular mechanism for contact-angle-dependent microtubule bundling.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Mechanical Signaling From ECMs to Nuclear Envelopes in Cell Transitions and Fate Decisions: A Review and Perspective on Adhesion-Niche-Based Anti-EndMT and Anti-EMT.Annals of biomedical engineering · 2026Review
- Assembly of Microtubule Tactoids Through Condensates of PRC1 Constructs.Biomolecules · 2026Article
- Thyme Oil Alleviates Cadmium-Induced Disturbances in Mitotic Activity, Cytoskeletal Organization and H3T3/H3S10 Phosphorylation inInternational journal of molecular sciences · 2026Article
- FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques.Plant methods · 2026Article
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Short coiled-coil proteins from plants and metazoans - the 'jacks of all trades'.The FEBS journal · 2025Review
- The N-terminal domain of COMPANION OF CELLULOSE SYNTHASE1 promotes microtubule array formation in Arabidopsis.Plant physiology · 2025Article
- Interplay of self-organization of microtubule asters and crosslinking protein condensates.PNAS nexus · 2023Article
- Intracellular infection by symbiotic bacteria requires the mitotic kinase AURORA1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Silver Nanoparticles Alter Microtubule Arrangement, Dynamics and Stress Phytohormone Levels.Plants (Basel, Switzerland) · 2022Article
- Mechanical stress effects on transcriptional regulation of genes encoding microtubule- and actin-associated proteins.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization.The Plant cell · 2021Article
- TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize.The Journal of cell biology · 2020Article
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Microtubule bundling by MAP65-1 protects against severing by inhibiting the binding of katanin.Molecular biology of the cell · 2019Article
- Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly.Biophysical journal · 2019Article
- TheFrontiers in plant science · 2018Article
- Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.Plant physiology · 2014Article
- The role of dynamic instability in microtubule organization.Frontiers in plant science · 2014Review
- MAPs: cellular navigators for microtubule array orientations in Arabidopsis.Plant cell reports · 2014Review
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4 authors.
Funding
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Abstract
Bundling of microtubules (MTs) is critical for the formation of complex MT arrays. In land plants, the interphase cortical MTs form bundles specifically following shallow-angle encounters between them. To investigate how cells select particular MT contact angles for bundling, we used an in vitro reconstitution approach consisting of dynamic MTs and the MT-cross-linking protein MAP65-1. We found that MAP65-1 binds to MTs as monomers and inherently targets antiparallel MTs for bundling. Dwell-time analysis showed that the affinity of MAP65-1 for antiparallel overlapping MTs is about three times higher than its affinity for single MTs and parallel overlapping MTs. We also found that purified MAP65-1 exclusively selects shallow-angle MT encounters for bundling, indicating that this activity is an intrinsic property of MAP65-1. Reconstitution experiments with mutant MAP65-1 proteins with different numbers of spectrin repeats within the N-terminal rod domain showed that the length of the rod domain is a major determinant of the range of MT bundling angles. The length of the rod domain also determined the distance between MTs within a bundle. Together, our data show that the rod domain of MAP65-1 acts both as a spacer and as a structural element that specifies the MT encounter angles that are conducive for bundling.
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