ArticleThe Annals of thoracic surgery2012
Human lung cancer cells grown on acellular rat lung matrix create perfusable tumor nodules.
Article in The Annals of thoracic surgery, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 75 citations in OpenAlex.
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- Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal.Bioactive materials · 2021Review
- Three-Dimensional Vascularized Lung Cancer-on-a-Chip with Lung Extracellular Matrix Hydrogels for In Vitro Screening.Cancers · 2021Article
- Drug delivery: Experiments, mathematical modelling and machine learning.Computers in biology and medicine · 2020Article
- Human-scale lung regeneration based on decellularized matrix scaffolds as a biologic platform.Surgery today · 2020Review
- Modeling the Tumor Microenvironment and Pathogenic Signaling in Bone Sarcoma.Tissue engineering. Part B, Reviews · 2020Review
- Decellularized Extracellular Matrix for Cancer Research.Materials (Basel, Switzerland) · 2019Review
- AnFrontiers in oncology · 2019Article
- Immune cells inhibit the tumor metastasis in the 4D cellular lung model by reducing the number of live circulating tumor cells.Scientific reports · 2018Article
- Acellular and Cellular Lung Model to Study Tumor Metastasis.Journal of visualized experiments : JoVE · 2018Article
- Small cell and non small cell lung cancer form metastasis on cellular 4D lung model.BMC cancer · 2018Article
- SR 11302, an AP-1 Inhibitor, Reduces Metastatic Lesion Formation in Ex Vivo 4D Lung Cancer Model.Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2017Article
- Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization.Scientific reports · 2017Article
- Targeting KRas-dependent tumour growth, circulating tumour cells and metastasis in vivo by clinically significant miR-193a-3p.Oncogene · 2017Article
- Native matrix-based human lung alveolar tissue model in vitro: studies of the reparatory actions of mesenchymal stem cells.Cytotechnology · 2017Article
- Engineering the pre-metastatic niche.Nature biomedical engineering · 2017Article
- Heralding a new paradigm in 3D tumor modeling.Biomaterials · 2016Review
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7 authors at 6 institutions in 1 country.
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Abstract
backgroundExtracellular matrix allows lung cancer to form its shape and grow. Recent studies on organ reengineering for orthotopic transplantation have provided a new avenue for isolating purified native matrix to use for growing cells. Whether human lung cancer cells grown in a decellularized rat lung matrix would create perfusable human lung cancer nodules was tested.
methodsRat lungs were harvested and native cells were removed using sodium dodecyl sulfate and Triton X-100 in a decellularization chamber to create a decellularized rat lung matrix. Human A549, H460, or H1299 lung cancer cells were placed into the decellularized rat lung matrix and grown in a customized bioreactor with perfusion of oxygenated media for 7 to 14 days.
resultsDecellularized rat lung matrix showed preservation of matrix architecture devoid of all rat cells. All three human lung cancer cell lines grown in the bioreactor developed tumor nodules with intact vasculature. Moreover, the lung cancer cells developed a pattern of growth similar to the original human lung cancer.
conclusionsOverall, this study shows that human lung cancer cells form perfusable tumor nodules in a customized bioreactor on a decellularized rat lung matrix created by a customized decellularization chamber. The lung cancer cells grown in the matrix had features similar to the original human lung cancer. This ex vivo model can be used potentially to gain a deeper understanding of the biologic processes involved in human lung cancer.
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