ArticleMicrobial biotechnology2012
A bacterial reporter panel for the detection and classification of antibiotic substances.
Article in Microbial biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Fighting the invader: strategies against intracellular bacteria.Frontiers in pharmacology · 2026Review
- Modulating effect of glutathione (GSH) on 2,4-dichlorophenoxyacetic acid (2,4-D) toxicity.Scientific reports · 2025Article
- Bioluminescent Microbial Bioreporters: A Personal Perspective.Biosensors · 2025Review
- The expression of different gene constructs in Escherichia coli SM lux biosensor after exposure to drugs.Scientific reports · 2024Article
- Determination of the Effect of Wastewater on the Biological Activity of Mixtures of Fluoxetine and Its Metabolite Norfluoxetine with Nalidixic and Caffeic Acids with Use ofMaterials (Basel, Switzerland) · 2023Article
- Antibiotic Susceptibility Testing with Raman Biosensing.Antibiotics (Basel, Switzerland) · 2022Review
- Overview on Strategies and Assays for Antibiotic Discovery.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project.Antibiotics (Basel, Switzerland) · 2022Article
- Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems.Frontiers in chemistry · 2022Review
- Smartphone-Based Whole-Cell Biosensor Platform Utilizing an Immobilization Approach on a Filter Membrane Disk for the Monitoring of Water Toxicants.Sensors (Basel, Switzerland) · 2020Article
- A Smartphone-Based Whole-Cell Array Sensor for Detection of Antibiotics in Milk.Sensors (Basel, Switzerland) · 2019Article
- Influence of Antibiotic Pressure on Five Plasmid-based Bioluminescent Gram-negative Bacterial Strains.Molecular imaging and biology · 2018Article
- Reconstructing promoter activity from Lux bioluminescent reporters.PLoS computational biology · 2017Article
- Techniques for Screening Translation Inhibitors.Antibiotics (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ever-growing use of pharmaceutical compounds, including antibacterial substances, poses a substantial pollution load on the environment. Such compounds can compromise water quality, contaminate soils, livestock and crops, enhance resistance of microorganisms to antibiotic substances, and hamper human health. We report the construction of a novel panel of genetically engineered Escherichia coli reporter strains for the detection and classification of antibiotic substances. Each of these strains harbours a plasmid that carries a fusion of a selected gene promoter to bioluminescence (luxCDABE) reporter genes and an alternative tryptophan auxotrophy-based non-antibiotic selection system. The bioreporter panel was tested for sensitivity and responsiveness to diverse antibiotic substances by monitoring bioluminescence as a function of time and of antibiotic concentrations. All of the tested antibiotics were detected by the panel, which displayed different response patterns for each substance. These unique responses were analysed by several algorithms that enabled clustering the compounds according to their functional properties, and allowed the classification of unknown antibiotic substances with a high degree of accuracy and confidence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.