Trial reportAmerican journal of reproductive immunology (New York, N.Y. : 1989)2012
Methylome of fetal and maternal monocytes and macrophages at the feto-maternal interface.
Trial report in American journal of reproductive immunology (New York, N.Y. : 1989), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 2 syntheses or guidelines pooled it, 99 citations in OpenAlex.
- Role of Transforming Growth Factor-β1 in Regulating Fetal-Maternal Immune Tolerance in Normal and Pathological Pregnancy.Frontiers in immunology · 2021Pooled it
- Gestational tissue transcriptomics in term and preterm human pregnancies: a systematic review and meta-analysis.BMC medical genomics · 2015Pooled it
- Endometrial cortisol level and its relationship with psychological stress, molecular tissue changes, and clinical outcomes in infertile women.Scientific reports · 2026Article
- Hofbauer Cells in Pregnancies Complicated by Gestational Diabetes Mellitus and Pathological Fetal Growth.American journal of reproductive immunology (New York, N.Y. : 1989) · 2026Article
- Inflammation at the maternal-fetal interface: a perspective on interacting risk factors for preterm birth in sub-Saharan African women living with HIV.Frontiers in immunology · 2026Review
- Isolation and characterization of Hofbauer cells from human term placentas.Frontiers in immunology · 2026Review
- Epigenetics of maternal-fetal interface immune microenvironment and placental related pregnancy complications.Frontiers in immunology · 2025Review
- Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies.Epigenetics · 2024Article
- Upregulation ofEpigenetics · 2024Article
- Gravidity influences distinct transcriptional profiles of maternal and fetal placental macrophages at term.Frontiers in immunology · 2024Article
- Decidual macrophages and Hofbauer cells in fetal growth restriction.Frontiers in immunology · 2024Review
- The N6-Methyladenosine RegulatorInternational journal of molecular sciences · 2022Article
- Article
- Immunological Changes in Pregnancy and Prospects of Therapeutic Pla-Xosomes in Adverse Pregnancy Outcomes.Frontiers in pharmacology · 2022Review
- Epigenetic regulation of pediatric and neonatal immune responses.Pediatric research · 2022Review
- Placental macrophages present distinct polarization pattern and effector functions depending on clinical onset of preeclampsia.Frontiers in immunology · 2022Article
- Impaired autophagy with augmented apoptosis in a Th1/Th2-imbalanced placental micromilieu is associated with spontaneous preterm birth.Frontiers in molecular biosciences · 2022Article
- Effects of Metabolism on Macrophage Polarization Under Different Disease Backgrounds.Frontiers in immunology · 2022Review
- Article
- Cytotrophoblasts suppress macrophage-mediated inflammation through a contact-dependent mechanism.American journal of reproductive immunology (New York, N.Y. : 1989) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
problemDecidual macrophages (dMφ) of the mother and placental macrophages (Hofbauer cells, HC) of the fetus are deployed at a critical location: the feto-maternal interface. This study was conducted to compare the DNA methylome of maternal and fetal monocytes, dMφ, and HC and thereby to determine the immunobiological importance of DNA methylation in pregnancy. METHOD OF STUDY: Paired samples were obtained from normal pregnant women at term not in labor and their neonates. Maternal monocytes (MMo) and fetal monocytes (FMo) were isolated from the peripheral blood of mothers and fetal cord blood, respectively. dMφ and HC were obtained from the decidua of fetal membranes and placentas, respectively. DNA methylation profiling was performed using the Illumina Infinium Human Methylation27 BeadChip. Quantitative real-time PCR and Western Blot were performed for validation experiments.
results(i) Significant differences in DNA methylation were found in each comparison (MMo versus FMo, 65 loci; dMφ versus HC, 266 loci; MMo versus dMφ, 199 loci; FMo versus HC, 1030 loci). (ii) Many of the immune response-related genes were hypermethylated in fetal cells (FMo and HC) compared to maternal cells (MMo and dMφ). (iii) Genes encoding markers of classical macrophage activation were hypermethylated, and genes encoding alternative macrophage activation were hypomethylated in dMφ and HC compared to MMo and FMo, respectively. (iv) mRNA expressions of DNMT1, DNMT3A, and DNMT3B were significantly lower in dMφ than in HC. (v) 5-azacytidine treatment increased expression of INCA1 in dMφ.
conclusionsThe findings herein indicate that DNA methylation patterns change during monocyte-macrophage differentiation at the feto-maternal interface. It is also suggested that DNA methylation is an important component of the biological machinery conferring an anti-inflammatory phenotype to macrophages at the feto-maternal interface.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.