ArticleG3 (Bethesda, Md.)2012
Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii.
Article in G3 (Bethesda, Md.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
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- Comparative Phenotyping of Two Commonly UsedPlants (Basel, Switzerland) · 2022Article
- The short flagella 1 (SHF1) gene inMolecular biology of the cell · 2022Article
- Discovery of photosynthesis genes through whole-genome sequencing of acetate-requiring mutants of Chlamydomonas reinhardtii.PLoS genetics · 2021Article
- Co-expression networks in Chlamydomonas reveal significant rhythmicity in batch cultures and empower gene function discovery.The Plant cell · 2021Article
- MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements.Plant physiology · 2018Article
- Identifying RNA splicing factors usingOpen biology · 2018Article
- Isolation, phenotypic characterization and genome wide analysis of aBiotechnology for biofuels · 2017Article
- Building a multipurpose insertional mutant library for forward and reverse genetics inPlant methods · 2017Article
- Volvox: A simple algal model for embryogenesis, morphogenesis and cellular differentiation.Developmental biology · 2016Review
- Mapping Challenging Mutations by Whole-Genome Sequencing.G3 (Bethesda, Md.) · 2016Article
- Article
- The Octatricopeptide Repeat Protein Raa8 Is Required for Chloroplast trans Splicing.Eukaryotic cell · 2015Article
- A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures.PLoS genetics · 2015Article
- Whole-Genome Resequencing Reveals Extensive Natural Variation in the Model Green Alga Chlamydomonas reinhardtii.The Plant cell · 2015Article
- MLT1 links cytoskeletal asymmetry to organelle placement in chlamydomonas.Cytoskeleton (Hoboken, N.J.) · 2015Article
- Fast forward genetics to identify mutations causing a high light tolerant phenotype in Chlamydomonas reinhardtii by whole-genome-sequencing.BMC genomics · 2015Article
- Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.Methods in cell biology · 2015Article
- A high-throughput fatty acid profiling screen reveals novel variations in fatty acid biosynthesis in Chlamydomonas reinhardtii and related algae.Eukaryotic cell · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Whole-genome sequencing (WGS) provides a new platform for the identification of mutations that produce a mutant phenotype. We used Illumina sequencing to identify the mutational profile of three Chlamydomonas reinhardtii mutant strains. The three strains have more than 38,000 changes from the reference genome. NG6 is aflagellate and maps to 269 kb with only one nonsynonymous change; the V(12)E mutation falls in the FLA8 gene. Evidence that NG6 is a fla8 allele comes from swimming revertants that are either true or pseudorevertants. NG30 is aflagellate and maps to 458 kb that has six nonsynonomous changes. Evidence that NG30 has a causative nonsense allele in IFT80 comes from rescue of the nonswimming phenotype with a fragment bearing only this gene. This gene has been implicated in Jeune asphyxiating thoracic dystrophy. Electron microscopy of ift80-1 (NG30) shows a novel basal body phenotype. A bar or cap is observed over the distal end of the transition zone, which may be an intermediate in preparing the basal body for flagellar assembly. In the acetate-requiring mutant ac17, we failed to find a nonsynonymous change in the 676 kb mapped region, which is incompletely assembled. In these strains, 43% of the changes occur on two of the 17 chromosomes. The excess on chromosome 6 surrounds the mating-type locus, which has numerous rearrangements and suppressed recombination, and the changes extend beyond the mating-type locus. Unexpectedly, chromosome 16 shows an unexplained excess of single nucleotide polymorphisms and indels. Overall, WGS in combination with limited mapping allows fast and accurate identification of point mutations in Chlamydomonas.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.