ArticleG3 (Bethesda, Md.)2011
High-Resolution SNP/CGH Microarrays Reveal the Accumulation of Loss of Heterozygosity in Commonly Used Candida albicans Strains.
Article in G3 (Bethesda, Md.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Evolution and strain diversity advance exploration ofmSphere · 2024Review
- Probing gene function inmSphere · 2024Article
- The C-terminal protein interaction domain of the chromatin reader Yaf9 is critical for pathogenesis ofmSphere · 2024Article
- Strain background ofMicrobiology (Reading, England) · 2024Article
- CRISPR-Cas9 Editing Induces Loss of Heterozygosity in the Pathogenic Yeast Candida parapsilosis.mSphere · 2022Article
- Genome plasticity driven by aneuploidy and loss of heterozygosity inMicrobial genomics · 2022Article
- Candida albicans commensalism in the oral mucosa is favoured by limited virulence and metabolic adaptation.PLoS pathogens · 2022Article
- A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization.Biosensors · 2021Article
- Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans.Journal, genetic engineering & biotechnology · 2021Article
- Factors that influence bidirectional long-tract homozygosis due to double-strand break repair in Candida albicans.Genetics · 2021Article
- Use of CRISPR-Cas9 To Target Homologous Recombination Limits Transformation-Induced Genomic Changes in Candida albicans.mSphere · 2020Article
- Virulence phenotypes result from interactions between pathogen ploidy and genetic background.Ecology and evolution · 2020Article
- The Impact of Gene Dosage and Heterozygosity on The Diploid PathobiontJournal of fungi (Basel, Switzerland) · 2019Review
- The Magnitude ofG3 (Bethesda, Md.) · 2019Article
- Aneuploidy Enables Cross-Adaptation to Unrelated Drugs.Molecular biology and evolution · 2019Article
- Whole genome sequencing of Trypanosoma cruzi field isolates reveals extensive genomic variability and complex aneuploidy patterns within TcII DTU.BMC genomics · 2018Article
- Rapid Phenotypic and Genotypic Diversification After Exposure to the Oral Host Niche inGenetics · 2018Article
- Gene flow contributes to diversification of the major fungal pathogen Candida albicans.Nature communications · 2018Article
- Gene and Chromosomal Copy Number Variations as an Adaptive Mechanism Towards a Parasitic Lifestyle in Trypanosomatids.Current genomics · 2018Review
- Modulation of the Fungal-Host Interaction by the Intra-Species Diversity of C. albicans.Pathogens (Basel, Switzerland) · 2018Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
Phenotypic diversity can arise rapidly through loss of heterozygosity (LOH) or by the acquisition of copy number variations (CNV) spanning whole chromosomes or shorter contiguous chromosome segments. In Candida albicans, a heterozygous diploid yeast pathogen with no known meiotic cycle, homozygosis and aneuploidy alter clinical characteristics, including drug resistance. Here, we developed a high-resolution microarray that simultaneously detects ∼39,000 single nucleotide polymorphism (SNP) alleles and ∼20,000 copy number variation loci across the C. albicans genome. An important feature of the array analysis is a computational pipeline that determines SNP allele ratios based upon chromosome copy number. Using the array and analysis tools, we constructed a haplotype map (hapmap) of strain SC5314 to assign SNP alleles to specific homologs, and we used it to follow the acquisition of loss of heterozygosity (LOH) and copy number changes in a series of derived laboratory strains. This high-resolution SNP/CGH microarray and the associated hapmap facilitated the phasing of alleles in lab strains and revealed detrimental genome changes that arose frequently during molecular manipulations of laboratory strains. Furthermore, it provided a useful tool for rapid, high-resolution, and cost-effective characterization of changes in allele diversity as well as changes in chromosome copy number in new C. albicans isolates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.