ArticleG3 (Bethesda, Md.)2011
Rediscovery by Whole Genome Sequencing: Classical Mutations and Genome Polymorphisms in Neurospora crassa.
Article in G3 (Bethesda, Md.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 71 citations in OpenAlex.
- Adaptive genetics reveals constraints on protein structure/function by evolving E. coli under constant nutrient limitation.BMC biology · 2025Article
- Unveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa.AMB Express · 2024Article
- Identification of a common secondary mutation in theMicrobiology spectrum · 2023Article
- The Role of Chromatin and Transcriptional Control in the Formation of Sexual Fruiting Bodies in Fungi.Microbiology and molecular biology reviews : MMBR · 2022Review
- Comparative Genomic Analysis Uncovers the Chloroplast Genome Variation and Phylogenetic Relationships ofBiomolecules · 2022Article
- Phenotype to genotype inCurrent research in microbial sciences · 2022Article
- Complete Genome Sequences and Genome-Wide Characterization ofMicrobiology spectrum · 2021Article
- Important role of melanin for fertility in the fungus Podospora anserina.G3 (Bethesda, Md.) · 2021Article
- The F-box protein geneProceedings of the National Academy of Sciences of the United States of America · 2021Article
- A global search for novel transcription factors impacting the Neurospora crassa circadian clock.G3 (Bethesda, Md.) · 2021Article
- Repeat-induced point mutation in Neurospora crassa causes the highest known mutation rate and mutational burden of any cellular life.Genome biology · 2020Article
- Whole Genome Sequencing Reveals Major Deletions in the Genome of M7, a Gamma Ray-Induced Mutant ofFrontiers in microbiology · 2020Article
- The Fungal Cell Death RegulatorJournal of fungi (Basel, Switzerland) · 2019Article
- A RID-like putative cytosine methyltransferase homologue controls sexual development in the fungus Podospora anserina.PLoS genetics · 2019Article
- The Genetics and Biochemistry of Cell Wall Structure and Synthesis inFrontiers in microbiology · 2019Review
- A-to-I RNA editing is developmentally regulated and generally adaptive for sexual reproduction inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Functional Profiling of Transcription Factor Genes inG3 (Bethesda, Md.) · 2017Article
- Article
- The ancestral levels of transcription and the evolution of sexual phenotypes in filamentous fungi.PLoS genetics · 2017Article
- Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era.Mycology · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Classical forward genetics has been foundational to modern biology, and has been the paradigm for characterizing the role of genes in shaping phenotypes for decades. In recent years, reverse genetics has been used to identify the functions of genes, via the intentional introduction of variation and subsequent evaluation in physiological, molecular, and even population contexts. These approaches are complementary and whole genome analysis serves as a bridge between the two. We report in this article the whole genome sequencing of eighteen classical mutant strains of Neurospora crassa and the putative identification of the mutations associated with corresponding mutant phenotypes. Although some strains carry multiple unique nonsynonymous, nonsense, or frameshift mutations, the combined power of limiting the scope of the search based on genetic markers and of using a comparative analysis among the eighteen genomes provides strong support for the association between mutation and phenotype. For ten of the mutants, the mutant phenotype is recapitulated in classical or gene deletion mutants in Neurospora or other filamentous fungi. From thirteen to 137 nonsense mutations are present in each strain and indel sizes are shown to be highly skewed in gene coding sequence. Significant additional genetic variation was found in the eighteen mutant strains, and this variability defines multiple alleles of many genes. These alleles may be useful in further genetic and molecular analysis of known and yet-to-be-discovered functions and they invite new interpretations of molecular and genetic interactions in classical mutant strains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.