ArticlePloS one2012
Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- Role of the TPR family protein VPA1365 in regulating type III secretion system 2 and virulence inApplied and environmental microbiology · 2025Article
- Influence of Urinary Urea Concentration on Hemolytic Activity and Cytotoxicity of Uropathogenic Morganella morganii Strain.Bulletin of experimental biology and medicine · 2024Article
- A coordinated attack by a bacterial secretion system and a small molecule drives prey specificity.Communications biology · 2024Article
- Features of Hemolysin Biosynthesis by Morganella morganii.Bulletin of experimental biology and medicine · 2023Article
- Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism inFrontiers in microbiology · 2022Article
- Molecular docking study of bio-inhibitors extracted from marine macro-alga Ulva fasciata against hemolysin protein of luminescence disease-causing Vibrio harveyi.Archives of microbiology · 2021Article
- Novel Insight into the Effects of CpxR onInternational journal of molecular sciences · 2021Article
- Characterization and Analysis of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in Pandemic and Non-PandemicMicroorganisms · 2021Article
- 4-Methylcytosine DNA modification is critical for global epigenetic regulation and virulence in the human pathogen Leptospira interrogans.Nucleic acids research · 2020Article
- Article
- Characterization of the hemolytic activity ofMicrobiology (Reading, England) · 2020Article
- Role of CpxR in Biofilm Development: Expression of Key Fimbrial, O-Antigen and Virulence Operons ofInternational journal of molecular sciences · 2019Article
- Alternative Sigma Factor RpoX Is a Part of the RpoE Regulon and Plays Distinct Roles in Stress Responses, Motility, Biofilm Formation, and Hemolytic Activities in the Marine Pathogen Vibrio alginolyticus.Applied and environmental microbiology · 2019Article
- Comparative Genome Analysis of UropathogenicFrontiers in cellular and infection microbiology · 2019Article
- YraP Contributes to Cell Envelope Integrity and Virulence of Salmonella enterica Serovar Typhimurium.Infection and immunity · 2018Article
- Discovery ofInternational journal of molecular sciences · 2018Article
- The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence inFrontiers in cellular and infection microbiology · 2018Article
- Studies of the extracytoplasmic function sigma factor PG0162 in Porphyromonas gingivalis.Molecular oral microbiology · 2016Article
- A σE-Mediated Temperature Gauge Controls a Switch from LuxR-Mediated Virulence Gene Expression to Thermal Stress Adaptation in Vibrio alginolyticus.PLoS pathogens · 2016Article
- Post-Genomic Analysis of Members of the Family Vibrionaceae.Microbiology spectrum · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the genus Vibrio are important marine and aquaculture pathogens. Hemolytic activity has been identified as a virulence factor in many pathogenic vibrios including V. cholerae, V. parahaemolyticus, V. alginolyticus, V. harveyi and V. vulnificus. We have used transposon mutagenesis to identify genes involved in the hemolytic activity of shrimp-pathogenic V. harveyi strain PSU3316. Out of 1,764 mutants screened, five mutants showed reduced hemolytic activity on sheep blood agar and exhibited virulence attenuation in shrimp (Litopenaeus vannamei). Mutants were identified by comparing transposon junction sequences to a draft of assembly of the PSU3316 genome. Surprisingly none of the disrupted open reading frames or gene neighborhoods contained genes annotated as hemolysins. The gene encoding RseB, a negative regulator of the sigma factor (σ(E)), was interrupted in 2 out of 5 transposon mutants, in addition, the transcription factor CytR, a threonine synthetase, and an efflux-associated cytoplasmic protein were also identified. Knockout mutations introduced into the rpoE operon at the rseB gene exhibited low hemolytic activity in sheep blood agar, and were 3-to 7-fold attenuated for colonization in shrimp. Comparison of whole cell extracted proteins in the rseB mutant (PSU4030) to the wild-type by 2-D gel electrophoresis revealed 6 differentially expressed proteins, including two down-regulated porins (OmpC-like and OmpN) and an upregulated protease (DegQ) which have been associated with σ(E) in other organisms. Our study is the first report linking hemolytic activity to the σ(E) regulators in pathogenic Vibrio species and suggests expression of this virulence-linked phenotype is governed by multiple regulatory pathways within the V. harveyi.
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