ArticlePloS one2012
Sample preparation and liquid chromatography-tandem mass spectrometry for multiple steroids in mammalian and avian circulation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Androgen Exhibits a Protective Role Against Focal Erosions in Murine TNF-induced Inflammatory Arthritis.Journal of the Endocrine Society · 2024Article
- A highly sensitive LC-MS/MS method for quantitative determination of 7 vitamin D metabolites in mouse brain tissue.Analytical and bioanalytical chemistry · 2023Article
- Stress Assessment of Wild Boar (Animals : an open access journal from MDPI · 2022Article
- Analysis of endogenous metabolites using multifunctional derivatization and capillary RPLC-MS.Analytical methods : advancing methods and applications · 2022Article
- Comparison of steroid and thyroid hormone concentrations in blood serum and plasma of captive tigers.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- Use of Sugar Cane Bagasse as Solid Extraction Phase Sorbent to Analyze Hormones from Industrial Effluent.ACS omega · 2022Article
- Rapidity and Precision of Steroid Hormone Measurement.Journal of clinical medicine · 2022Review
- Introduction of gloved hand to cage induces 22-kHz ultrasonic vocalizations in male albino rats.PloS one · 2022Article
- Profiling of Steroid Metabolic Pathways in Human Plasma by GC-MS/MS Combined with Microwave-Assisted Derivatization for Diagnosis of Gastric Disorders.International journal of molecular sciences · 2021Article
- How technical progress reshaped behavioral neuroendocrinology during the last 50 years… and some methodological remarks.Hormones and behavior · 2020Review
- Hair cortisol analyses in different mammal species: choosing the wrong assay may lead to erroneous results.Conservation physiology · 2020Article
- Pubertal development of estradiol-induced hypothalamic progesterone synthesis.Hormones and behavior · 2019Article
- A sensitive and robust UPLC-MS/MS method for quantitation of estrogens and progestogens in human serum.Contraception · 2019Article
- Prediction and Understanding of Resilience in Albertan Families: Longitudinal Study of Disaster Responses (PURLS) - Protocol.Frontiers in psychiatry · 2019Article
- Qiviut cortisol in muskoxen as a potential tool for informing conservation strategies.Conservation physiology · 2017Article
- Sex steroid profiles and pair-maintenance behavior of captive wild-caught zebra finches (Taeniopygia guttata).Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2016Article
- Feather corticosterone reveals stress associated with dietary changes in a breeding seabird.Ecology and evolution · 2015Article
- Phthalate esters affect maturation and function of primate testis tissue ectopically grafted in mice.Molecular and cellular endocrinology · 2014Article
- Article
- Transport versus on-farm slaughter of bison: physiological stress, animal welfare, and avoidable trim losses.The Canadian veterinary journal = La revue veterinaire canadienne · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Blood samples from wild mammals and birds are often limited in volume, allowing researchers to quantify only one or two steroids from a single sample by immunoassays. In addition, wildlife serum or plasma samples are often lipemic, necessitating stringent sample preparation. Here, we validated sample preparation for simultaneous liquid chromatography--tandem mass spectrometry (LC-MS/MS) quantitation of cortisol, corticosterone, 11-deoxycortisol, dehydroepiandrosterone (DHEA), 17β-estradiol, progesterone, 17α-hydroxyprogesterone and testosterone from diverse mammalian (7 species) and avian (5 species) samples. Using 100 µL of serum or plasma, we quantified (signal-to-noise (S/N) ratio ≥ 10) 4-7 steroids depending on the species and sample, without derivatization. Steroids were extracted from serum or plasma using automated solid-phase extraction where samples were loaded onto C18 columns, washed with water and hexane, and then eluted with ethyl acetate. Quantitation by LC-MS/MS was done in positive ion, multiple reaction-monitoring (MRM) mode with an atmospheric pressure chemical ionization (APCI) source and heated nebulizer (500°C). Deuterated steroids served as internal standards and run time was 15 minutes. Extraction recoveries were 87-101% for the 8 analytes, and all intra- and inter-run CVs were ≤ 8.25%. This quantitation method yields good recoveries with variable lipid-content samples, avoids antibody cross-reactivity issues, and delivers results for multiple steroids. Thus, this method can enrich datasets by providing simultaneous quantitation of multiple steroids, and allow researchers to reimagine the hypotheses that could be tested with their volume-limited, lipemic, wildlife samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.