ArticlePloS one2012
Isothermal amplification using a chemical heating device for point-of-care detection of HIV-1.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed, 131 citations in OpenAlex.
- Rapid, low-cost, and portable molecular detection of HIV-1 subtype B and HCV 1a directly from blood.Microbiology spectrum · 2026Article
- A self-heating, multi-channel slider cassette for innovative point-of-care molecular diagnostics from whole blood samples.Biosensors & bioelectronics · 2025Article
- 3D-Printed CRISPR-based detection system powered by a reusable handwarmer.Biomedical microdevices · 2025Article
- A new approach for the detection of genetic alterations utilizing modified loop-mediated isothermal amplification reaction (LAMP).Scientific reports · 2025Article
- Compact multiplex PCR device for HIV-1 and HIV-2 viral load determination from finger-prick whole blood in resource-limited settings.Biosensors & bioelectronics · 2025Article
- Point-of-Care Diagnostics Using Self-heating Elements from Smart Food Packaging: Moving Towards Instrument-Free Nucleic Acid-Based Detection.Molecular diagnosis & therapy · 2025Review
- A handheld HIV detection platform using paper-based sample preparation and real-time isothermal amplification.Microsystems & nanoengineering · 2024Article
- Chemical Heating for Minimally Instrumented Point-of-Care (POC) Molecular Diagnostics.Biosensors · 2024Review
- Adaptation of a Model Spike Aptamer for Isothermal Amplification-Based Sensing.Sensors (Basel, Switzerland) · 2024Article
- Isothermal Amplification Using Temperature-Controlled Frequency Mixing Magnetic Detection-Based Portable Field-Testing Platform.Sensors (Basel, Switzerland) · 2024Article
- Development and evaluation of a high-sensitivity RT-PCR lateral flow assay for early detection of HIV-1 infection.Heliyon · 2024Article
- Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP).Scientific reports · 2024Article
- Compact Point-of-Care Device for Self-Administered HIV Viral Load Tests from Whole Blood.ACS sensors · 2023Article
- Two-step method for rapid isolation of genomic DNA and validation of R81T insecticide resistance mutation inSaudi journal of biological sciences · 2023Article
- Validation of quantitative loop-mediated isothermal amplification assay using a fluorescent distance-based paper device for detection of Escherichia coli in urine.Scientific reports · 2023Article
- Review of HIV Self Testing Technologies and Promising Approaches for the Next Generation.Biosensors · 2023Review
- Strategies for the Voltammetric Detection of Loop-Mediated Isothermal Amplification.Micromachines · 2023Review
- Rapid and visual identification of HIV-1 using reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticle-based lateral flow assay platform.Frontiers in microbiology · 2023Article
- Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.PloS one · 2023Article
- Distance-based paper device using combined SYBR safe and gold nanoparticle probe LAMP assay to detect Leishmania among patients with HIV.Scientific reports · 2022Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements. The availability of a rapid NAAT with applicability for resource-limited or point-of-care (POC) settings would fill a great need in HIV diagnostics, allowing for timely diagnosis or confirmation of infection status, as well as facilitating the diagnosis of acute infection, screening and evaluation of infants born to HIV-infected mothers. Isothermal amplification methods, such as reverse-transcription, loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for POC settings, since they are typically quicker, easier to perform, and allow for integration into low-tech, portable heating devices. METHODOLOGY/SIGNIFICANT
findingsIn this study, we evaluated the HIV-1 RT-LAMP assay using portable, non-instrumented nucleic acid amplification (NINA) heating devices that generate heat from the exothermic reaction of calcium oxide and water. The NINA heating devices exhibited stable temperatures throughout the amplification reaction and consistent amplification results between three separate devices and a thermalcycler. The performance of the NINA heaters was validated using whole blood specimens from HIV-1 infected patients.
conclusionThe RT-LAMP isothermal amplification method used in conjunction with a chemical heating device provides a portable, rapid and robust NAAT platform that has the potential to facilitate HIV-1 testing in resource-limited settings and POC.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.