ArticlePloS one2012
F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
- Biodegradation of Aflatoxins by Four Novel Environmental Bacterial Isolates: A Comparative Genomic and Molecular Docking-Proof-of-Concept Study.Foods (Basel, Switzerland) · 2026Article
- Structure Elucidation and Toxicity Analyses of the Degradation Products of Aflatoxin BJournal of fungi (Basel, Switzerland) · 2026Article
- Study on the Enrichment of the Main Active Components inFoods (Basel, Switzerland) · 2025Article
- Enzymatic detoxification of major mycotoxins: current status, challenges, and future prospective.Mycotoxin research · 2025Review
- A Review of Research Progress on the Microbial or Enzymatic Degradation and Mechanism of Aflatoxin B1.Journal of microbiology and biotechnology · 2025Review
- Network pharmacology-based research on the effect of angelicin on osteosarcoma and the underlying mechanism.Aging · 2023Article
- Article
- On the diversity of FProteins · 2021Article
- Cofactor F420: an expanded view of its distribution, biosynthesis and roles in bacteria and archaea.FEMS microbiology reviews · 2021Article
- Potency boost of aProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Aflatoxins: Producing-Molds, Structure, Health Issues and Incidence in Southeast Asian and Sub-Saharan African Countries.International journal of environmental research and public health · 2020Review
- Predicting nitroimidazole antibiotic resistance mutations in Mycobacterium tuberculosis with protein engineering.PLoS pathogens · 2020Article
- Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action.Molecules (Basel, Switzerland) · 2019Review
- Recombinant Aflatoxin-Degrading FToxins · 2019Article
- Coenzyme FJournal of bacteriology · 2018Article
- Discovery and characterization of an FApplied microbiology and biotechnology · 2017Article
- Mycobacterial FFrontiers in microbiology · 2017Article
- The methanogenic redox cofactor FThe ISME journal · 2017Article
- The Redox Cofactor FApplied and environmental microbiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two classes of F(420)-dependent reductases (FDR-A and FDR-B) that can reduce aflatoxins and thereby degrade them have previously been isolated from Mycobacterium smegmatis. One class, the FDR-A enzymes, has up to 100 times more activity than the other. F(420) is a cofactor with a low reduction potential that is largely confined to the Actinomycetales and some Archaea and Proteobacteria. We have heterologously expressed ten FDR-A enzymes from diverse Actinomycetales, finding that nine can also use F(420)H(2) to reduce aflatoxin. Thus FDR-As may be responsible for the previously observed degradation of aflatoxin in other Actinomycetales. The one FDR-A enzyme that we found not to reduce aflatoxin belonged to a distinct clade (herein denoted FDR-AA), and our subsequent expression and analysis of seven other FDR-AAs from M. smegmatis found that none could reduce aflatoxin. Certain FDR-A and FDR-B enzymes that could reduce aflatoxin also showed activity with coumarin and three furanocoumarins (angelicin, 8-methoxysporalen and imperatorin), but none of the FDR-AAs tested showed any of these activities. The shared feature of the compounds that were substrates was an α,β-unsaturated lactone moiety. This moiety occurs in a wide variety of otherwise recalcitrant xenobiotics and antibiotics, so the FDR-As and FDR-Bs may have evolved to harness the reducing power of F(420) to metabolise such compounds. Mass spectrometry on the products of the FDR-catalyzed reduction of coumarin and the other furanocoumarins shows their spontaneous hydrolysis to multiple products.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.