ArticleReproductive sciences (Thousand Oaks, Calif.)2012
In vitro culture of mouse embryos reduces differential gene expression between inner cell mass and trophectoderm.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- The Fallopian tube: Biosensor and dynamic regulator of preimplantation development.PNAS nexus · 2026Review
- Screening of nontoxic dyes for improved visualization and success rate in mouse embryo transfer.Laboratory animal research · 2025Article
- Cryopreservation, cryoprotectants, and potential risk of epigenetic alteration.Journal of assisted reproduction and genetics · 2024Review
- miRNAs in Follicular and Oviductal Fluids Support Global DNA Demethylation in Early-Stage Embryos.International journal of molecular sciences · 2024Article
- Long-Term Effects of ART on the Health of the Offspring.International journal of molecular sciences · 2023Review
- Fertility treatment increases the risk of preterm birth independent of multiple gestations.F&S reports · 2023Article
- Developmental competence of IVF and SCNT goat embryos is improved by inhibition of canonical WNT signaling.PloS one · 2023Article
- Article
- The global gene expression outline of the bovine blastocyst: reflector of environmental conditions and predictor of developmental capacity.BMC genomics · 2021Article
- In Vitro Fertilisation of Mouse Oocytes in L-Proline and L-Pipecolic Acid Improves Subsequent Development.Cells · 2021Article
- Histone Modifications of H3K4me3, H3K9me3 and Lineage Gene Expressions in Chimeric Mouse Embryo.Cell journal · 2020Article
- In vitro fertilization and embryo transfer alter human placental function through trophoblasts in early pregnancy.Molecular medicine reports · 2020Article
- Past, Present, and Future Strategies for Enhanced Assessment of Embryo's Genome and Reproductive Competence in Women of Advanced Reproductive Age.Frontiers in endocrinology · 2019Review
- Effects of Gravity, Microgravity or Microgravity Simulation on Early Mammalian Development.Stem cells and development · 2018Review
- Global, Survival, and Apoptotic Transcriptome during Mouse and Human Early Embryonic Development.BioMed research international · 2018Article
- Sex differences in rat placental development: from pre-implantation to late gestation.Biology of sex differences · 2017Article
- Transcriptome profiling of bovine inner cell mass and trophectoderm derived from in vivo generated blastocysts.BMC developmental biology · 2015Article
- Human stem cells from single blastomeres reveal pathways of embryonic or trophoblast fate specification.Development (Cambridge, England) · 2015Article
- The placenta: phenotypic and epigenetic modifications induced by Assisted Reproductive Technologies throughout pregnancy.Clinical epigenetics · 2015Review
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Differences in gene expression and imprinting have been reported, comparing in vivo versus in vitro generated preimplantation embryos. Furthermore, mouse studies have shown that placenta development is altered following in vitro culture. However, the molecular mechanisms underlying these findings are unknown. We therefore isolated trophectoderm (TE) and inner cell mass (ICM) cells from in vivo and in vitro fertilization (IVF) embryos and evaluated their transcriptome using microarrays. We found that the transcriptomes of in vitro produced ICM and TE cells showed remarkably few differences compared to ICM and TE cells of in vivo generated embryos. In vitro fertilization embryos showed a reduced number of TE cells compared to in vivo embryos. In addition, TE of IVF embryos showed significant downregulation of solute transporter genes and of genes involved in placenta formation (Eomesodermin, Socs3) or implantation (Hbegf). In summary, IVF and embryo culture significantly affects the transcriptome of ICM and TE cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.