ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2012
Map-based cloning of a recessive genic male sterility locus in Brassica napus L. and development of its functional marker.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Towards premium rapeseed oil: leveraging allelic variation in BnaFAD2.A5, BnaFAD3.A4 and BnaFAD3.C4 for molecular design breeding.BMC plant biology · 2026Article
- Exploration of the molecular mechanism behind a novel natural genic male-sterile mutation of 1205A in Brassica napus.BMC plant biology · 2025Article
- Fertile grounds: exploring male sterility in cotton and its marker development.Molecular biology reports · 2024Review
- Genome-Wide Identification of the Cytochrome P450 Superfamily Genes and Targeted Editing ofPlants (Basel, Switzerland) · 2023Article
- Recent advancements in the breeding of sorghum crop: current status and future strategies for marker-assisted breeding.Frontiers in genetics · 2023Review
- Two wrongs make a right: heat stress reversion of a male-sterile Brassica napus line.Journal of experimental botany · 2022Article
- Global transcriptome analysis reveals potential genes associated with genic male sterility of rapeseed (Frontiers in plant science · 2022Article
- Fine mapping and candidate gene analysis of a seed glucosinolate content QTL, qGSL-C2, in rapeseed (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Mapping of the male sterile mutant gene ftms in Brassica rapa L. ssp. pekinensis via BSR-Seq combined with whole-genome resequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Identification of candidate genes underlying genic male-sterile msc-1 locus via genome resequencing in Capsicum annuum L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Fine mapping of the genic male-sterile msTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Map-based cloning reveals the complex organization of the BnRf locus and leads to the identification of BnRf(b), a male sterility gene, in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Common bean reaction to angular leaf spot comprises transcriptional modulation of genes in the ALS10.1 QTL.Frontiers in plant science · 2015Article
- Construction and characterization of a BAC library from the Coffea arabica genotype Timor Hybrid CIFC 832/2.Genetica · 2013Article
- A triallelic genetic male sterility locus in Brassica napus: an integrative strategy for its physical mapping and possible local chromosome evolution around it.Annals of botany · 2013Article
- Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
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7 authors.
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Abstract
We previously mapped one male-sterile gene (Bnms3) from an extensively used recessive genic male sterility line (9012AB) in Brassica napus to a 0.14-cM genomic region. In this study, two highly homologous BAC contigs possibly containing the candidate BnMs3 gene were identified using a map-based cloning strategy. A BnMs3-linked SCAR marker (DM1) capable of differentiating the subgenomes between B. rapa and the B. oleracea aided mapping of BnMs3 on the contig derived from the B. napus chromosome C9. One representative BAC clone was sequenced from each of the two contigs and resulted in a larger number of markers according to the sequence difference between the two clones. To isolate BnMs3, these markers were then analyzed in another two BC(1) populations with different genetic backgrounds. This assay allowed for a delimitation of the mutated functional region of BnMs3 to a 9.3-kb DNA fragment. Gene prediction suggested that one complete open reading frame (ORF, ORF2) and partial CDS fragments of ORF1 and ORF3 reside in this fragment. Sequence comparison and genetic transformation eventually indicated that ORF1 (designated as BnaC9.Tic40), an analogue of the Arabidopsis gene AT5G16620 which encodes a translocon of the inner envelope of chloroplasts 40 (Tic40), is the only candidate gene of BnMs3. Furthermore, two distinct mutation types in ORF1 both causing the male-sterile phenotype were individually revealed from 9012A and the temporary maintainer line T45. The molecular mechanism of this male sterility as well as the application of BnMs3-associated functional and cosegregated markers in true breeding programs was also discussed.
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