ArticleMolecular biotechnology2013
Enhanced adenovirus transduction of hMSCs using 3D hydrogel cell carriers.
Article in Molecular biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Application of Alginate Hydrogels for Next-Generation Articular Cartilage Regeneration.International journal of molecular sciences · 2022Review
- Ex Vivo Systems to Study Chondrogenic Differentiation and Cartilage Integration.Journal of functional morphology and kinesiology · 2021Review
- Biomaterial-Guided Gene Delivery for Musculoskeletal Tissue Repair.Tissue engineering. Part B, Reviews · 2017Review
- Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model.Advanced healthcare materials · 2015Article
- Gene therapy approaches to regenerating the musculoskeletal system.Nature reviews. Rheumatology · 2015Review
- Native, living tissues as cell seeded scaffolds.Annals of biomedical engineering · 2015Review
- Article
- Using genes to facilitate the endogenous repair and regeneration of orthopaedic tissues.International orthopaedics · 2014Review
- Tissue-engineered cartilage with inducible and tunable immunomodulatory properties.Biomaterials · 2014Article
- Advances in regenerative orthopedics.Mayo Clinic proceedings · 2013Article
- Hydrogels for lentiviral gene delivery.Expert opinion on drug delivery · 2013Review
- Cyclic-RGD peptides increase the adenoviral transduction of human mesenchymal stem cells.Stem cells and development · 2013Article
- Susceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogels are increasingly being investigated as a means to implant cells for tissue engineering. One way to further enhance the repair response would be to combine the hydrogel cell carrier with gene transfer. Gene therapy, using adenoviral vectors, is an effective way to provide transient delivery of bioactive factors. However, current protocols require further optimization, especially if they are to be transferred into the clinic. This study opted to compare the efficiency of protocols for standard two-dimensional (2D) versus three-dimensional (3D), adenoviral-mediated, transduction of human mesenchymal stem cells. Two different multiplicities of infection were tested. After encapsulation in fibrin, alginate or agarose, cells were cultured for 28 days. Transduction in 3D showed a much higher efficiency, compared to standard 2D transduction protocols. In 3D, the amount of transgene produced was significantly higher, for every condition investigated. Furthermore, transduction in 3D does not require a cell culture step and can be conducted within the operating theatre. In conclusion, it was demonstrated that 3D transduction, using adenoviral vectors, is superior to standard transduction protocols in 2D. It therefore, might help increasing its administration in tissue engineering and clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.