ArticleInorganic chemistry2012
Reaction of metal-binding ligands with the zinc proteome: zinc sensors and N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine.
Article in Inorganic chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Chelation-Driven Dual-Interface Regulation for Long-Life Aqueous Zn-IAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- TPEN-Advanced Metal Chelator: From Characterization to Biomedical Applications.Molecules (Basel, Switzerland) · 2026Review
- Correlative imaging reveals metal dyshomeostasis and altered zinc coordination environments in a pre-clinical Type 2 diabetes model.Metallomics : integrated biometal science · 2026Article
- Targeting Biometals in Alzheimer's Disease with Metal Chelating Agents Including Coumarin Derivatives.CNS drugs · 2024Review
- Mobile zinc as a modulator of sensory perception.FEBS letters · 2023Review
- Silymarin Dehydroflavonolignans Chelate Zinc and Partially Inhibit Alcohol Dehydrogenase.Nutrients · 2021Article
- Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Interrogating Intracellular Zinc Chemistry with a Long Stokes Shift Zinc Probe ZincBY-4.Journal of the American Chemical Society · 2019Article
- Zinc-Chelating Small Molecules Preferentially Accumulate and Function within Pancreatic β Cells.Cell chemical biology · 2019Article
- Agonist-Evoked Increases in Intra-Platelet Zinc Couple to Functional Responses.Thrombosis and haemostasis · 2019Article
- Proteomic High Affinity ZnInternational journal of molecular sciences · 2017Review
- Zinc Selectively Blocks Neurosteroid-Sensitive Extrasynaptic δGABAA Receptors in the Hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Newport Green, a fluorescent sensor of weakly bound cellular Zn(2+): competition with proteome for Zn(2).Metallomics : integrated biometal science · 2016Article
- The Critical Roles of Zinc: Beyond Impact on Myocardial Signaling.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2015Review
- Article
- Elemental and chemically specific X-ray fluorescence imaging of biological systems.Chemical reviews · 2014Review
- Native SDS-PAGE: high resolution electrophoretic separation of proteins with retention of native properties including bound metal ions.Metallomics : integrated biometal science · 2014Article
- Fluorescent sensors for measuring metal ions in living systems.Chemical reviews · 2014Review
- Zn(II)-coordination modulated ligand photophysical processes - the development of fluorescent indicators for imaging biological Zn(II) ions.RSC advances · 2014Article
- Tris(2-pyridylmethyl)amine (TPA) as a membrane-permeable chelator for interception of biological mobile zinc.Metallomics : integrated biometal science · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The commonly used Zn(2+) sensors 6-methoxy-8-p-toluenesulfonamidoquinoline (TSQ) and Zinquin have been shown to image zinc proteins as a result of the formation of sensor-zinc-protein ternary adducts not Zn(TSQ)(2) or Zn(Zinquin)(2) complexes. The powerful, cell-permeant chelating agent N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) is also used in conjunction with these and other Zn(2+) sensors to validate that the observed fluorescence enhancement seen with the sensors depends on intracellular interaction with Zn(2+). We demonstrated that the kinetics of the reaction of TPEN with cells pretreated with TSQ or Zinquin was not consistent with its reaction with Zn(TSQ)(2) or Zn(Zinquin)(2). Instead, TPEN and other chelating agents extract between 25 and 35% of the Zn(2+) bound to the proteome, including zinc(2+) from zinc metallothionein, and thereby quench some, but not all, of the sensor-zinc-protein fluorescence. Another mechanism in which TPEN exchanges with TSQ or Zinquin to form TPEN-zinc-protein adducts found support in the reactions of TPEN with Zinquin-zinc-alcohol dehydrogenase. TPEN also removed one of the two Zn(2+) ions per monomer from zinc-alcohol dehydrogenase and zinc-alkaline phosphatase, consistent with its ligand substitution reactivity with the zinc proteome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.