ArticleMolecular biology of the cell2012
The endosomal adaptor protein APPL1 impairs the turnover of leading edge adhesions to regulate cell migration.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancer.The EMBO journal · 2024Article
- APPL1 Is a Prognostic Biomarker and Correlated with Treg Cell Infiltration via Oxygen-Consuming Metabolism in Renal Clear Cell Carcinoma.Oxidative medicine and cellular longevity · 2023Article
- Rapid whole cell imaging reveals a calcium-APPL1-dynein nexus that regulates cohort trafficking of stimulated EGF receptors.Communications biology · 2021Article
- Mutant p53 amplifies a dynamin-1/APPL1 endosome feedback loop that regulates recycling and migration.The Journal of cell biology · 2019Article
- Arf GAPs and molecular motors.Small GTPases · 2019Review
- ARAP2 inhibits Akt independently of its effects on focal adhesions.Biology of the cell · 2018Article
- α5β1 integrin trafficking and Rac activation are regulated by APPL1 in a Rab5-dependent manner to inhibit cell migration.Journal of cell science · 2018Article
- Article
- Integration of GPCR Signaling and Sorting from Very Early Endosomes via Opposing APPL1 Mechanisms.Cell reports · 2017Article
- APPL1 is a multifunctional endosomal signaling adaptor protein.Biochemical Society transactions · 2017Review
- MYO6 Regulates Spatial Organization of Signaling Endosomes Driving AKT Activation and Actin Dynamics.Cell reports · 2017Article
- Appl1 and Appl2 are Expendable for Mouse Development But Are Essential for HGF-Induced Akt Activation and Migration in Mouse Embryonic Fibroblasts.Journal of cellular physiology · 2016Article
- On the move: endocytic trafficking in cell migration.Cellular and molecular life sciences : CMLS · 2015Review
- Automated analysis of cell-matrix adhesions in 2D and 3D environments.Scientific reports · 2015Article
- Altered endosome biogenesis in prostate cancer has biomarker potential.Molecular cancer research : MCR · 2014Article
- The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct endosomal compartments that regulate integrin α5β1 traffic.The Journal of biological chemistry · 2014Article
- Myosin VI and its cargo adaptors - linking endocytosis and autophagy.Journal of cell science · 2013Review
- Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt.Nature protocols · 2013Article
- Essential role of class II phosphatidylinositol-3-kinase-C2α in sphingosine 1-phosphate receptor-1-mediated signaling and migration in endothelial cells.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Cell migration is a complex process that requires the integration of signaling events that occur in distinct locations within the cell. Adaptor proteins, which can localize to different subcellular compartments, where they bring together key signaling proteins, are emerging as attractive candidates for controlling spatially coordinated processes. However, their function in regulating cell migration is not well understood. In this study, we demonstrate a novel role for the adaptor protein containing a pleckstrin-homology (PH) domain, phosphotyrosine-binding (PTB) domain, and leucine zipper motif 1 (APPL1) in regulating cell migration. APPL1 impairs migration by hindering the turnover of adhesions at the leading edge of cells. The mechanism by which APPL1 regulates migration and adhesion dynamics is by inhibiting the activity of the serine/threonine kinase Akt at the cell edge and within adhesions. In addition, APPL1 significantly decreases the tyrosine phosphorylation of Akt by the nonreceptor tyrosine kinase Src, which is critical for Akt-mediated cell migration. Thus, our results demonstrate an important new function for APPL1 in regulating cell migration and adhesion turnover through a mechanism that depends on Src and Akt. Moreover, our data further underscore the importance of adaptor proteins in modulating the flow of information through signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.