ArticleJournal of virology2012
The extended cell panel assay characterizes the relationship of prion strains RML, 79A, and 139A and reveals conversion of 139A to 79A-like prions in cell culture.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Strain-specific propagation of variant Creutzfeldt-Jakob disease prions in humanized neural cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The molecular determinants of a universal prion acceptor.PLoS pathogens · 2024Article
- Genetically engineered cellular models of prion propagation.Cell and tissue research · 2023Review
- Prion Strains Differ in Susceptibility to Photodynamic Oxidation.Molecules (Basel, Switzerland) · 2022Article
- From Cell Culture to Organoids-Model Systems for Investigating Prion Strain Characteristics.Biomolecules · 2021Review
- The role of prion strain diversity in the development of successful therapeutic treatments.Progress in molecular biology and translational science · 2020Review
- Engineering a murine cell line for the stable propagation of hamster prions.The Journal of biological chemistry · 2019Article
- Different 2-Aminothiazole Therapeutics Produce Distinct Patterns of Scrapie Prion Neuropathology in Mouse Brains.The Journal of pharmacology and experimental therapeutics · 2015Article
- Review
- Article
- Unique drug screening approach for prion diseases identifies tacrolimus and astemizole as antiprion agents.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Post-translational changes to PrP alter transmissible spongiform encephalopathy strain properties.The EMBO journal · 2013Article
- Heterogeneity of the Abnormal Prion Protein (PrPSc) of the Chandler Scrapie Strain.Pathogens (Basel, Switzerland) · 2013Article
- Acquisition of drug resistance and dependence by prions.PLoS pathogens · 2013Article
- Strain-specific role of RNAs in prion replication.Journal of virology · 2012Article
- Propagation of RML prions in mice expressing PrP devoid of GPI anchor leads to formation of a novel, stable prion strain.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Three commonly used isolates of murine prions, 79A, 139A, and RML, were derived from the so-called Chandler isolate, which was obtained by propagating prions from scrapie-infected goat brain in mice. RML is widely believed to be identical with 139A; however, using the extended cell panel assay (ECPA), we here show that 139A and RML isolates are distinct, while 79A and RML could not be distinguished. We undertook to clone 79A and 139A prions by endpoint dilution in murine neuroblastoma-derived PK1 cells. Cloned 79A prions, when returned to mouse brain, were unchanged and indistinguishable from RML by ECPA. However, 139A-derived clones, when returned to brain, yielded prions distinct from 139A and similar to 79A and RML. Thus, when 139A prions were transferred to PK1 cells, 79A/RML-like prions, either present as a minor component in the brain 139A population or generated by mutation in the cells, were selected and, after being returned to brain, were the major if not only component of the population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.