ArticleJournal of experimental botany2012
Identification and evaluation of ω-3 fatty acid desaturase genes for hyperfortifying α-linolenic acid in transgenic rice seed.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed.
- Metabolic engineering of cereal lipids: from omega-3 fatty acids to wax esters and pheromones.Advanced biotechnology · 2026Review
- Molecular Basis of Lipid Metabolism inPlants (Basel, Switzerland) · 2024Review
- Profiling and Improvement of Grain Quality Traits for Consumer Preferable Basmati Rice in the United States.Plants (Basel, Switzerland) · 2024Review
- Biological Roles of Lipids in Rice.International journal of molecular sciences · 2024Review
- Article
- Genome-Wide Association Study Identifies a Plant-Height-Associated GeneInternational journal of molecular sciences · 2023Article
- Overexpression of the Purple Perilla (International journal of molecular sciences · 2023Article
- Genome-wide association study reveals useful QTL and genes controlling the fatty acid composition in rice bran oil using Vietnamese rice landraces.Functional & integrative genomics · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- A near complete genome assembly of chia assists in identification of key fatty acid desaturases in developing seeds.Frontiers in plant science · 2023Article
- Evidence and Impacts of Nanoplastic Accumulation on Crop Grains.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Natural Variation of Fatty Acid Desaturase Gene Affects Linolenic Acid Content and Starch Pasting Viscosity in Rice Grains.International journal of molecular sciences · 2022Article
- qFC6, a major gene for crude fat content and quality in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Genes and Their Molecular Functions Determining Seed Structure, Components, and Quality of Rice.Rice (New York, N.Y.) · 2022Review
- Increase in alpha-linolenic acid content by simultaneous expression of fatty acid metabolism genes in Sesame (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Overexpression ofGenes · 2022Article
- In Silico Analysis of Fatty Acid Desaturases Structures inInternational journal of molecular sciences · 2021Article
- Article
- Overexpression ofFrontiers in plant science · 2021Article
- Endoplasmic reticulum retention signaling and transmembrane channel proteins predicted for oilseed ω3 fatty acid desaturase 3 (FAD3) genes.Functional & integrative genomics · 2020Article
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5 authors.
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Abstract
α-Linolenic acid (ALA) deficiency and a skewed of ω6:ω3 fatty acid ratio in the diet are a major explanation for the prevalence of cardiovascular diseases and inflammatory/autoimmune diseases. There is a need to enhance the ALA content and to reduce the ratio of linoleic acid (LA) to ALA. Six ω-3 (Δ-15) fatty acid desaturase (FAD) genes were cloned from rice and soybean. The subcellular localizations of the proteins were identified. The FAD genes were introduced into rice under the control of an endosperm-specific promoter, GluC, or a Ubi-1 promoter to evaluate their potential in increasing the ALA content in seeds. The ALA contents in the seeds of endoplasmic reticulum (ER)-localized GmFAD3-1 and OsFAD3 overexpression lines increased from 0.36 mg g⁻¹ to 8.57 mg g⁻¹ and 10.06 mg g⁻¹, respectively, which was 23.8- and 27.9-fold higher than that of non-transformants. The trait of high ALA content was stably inheritable over three generations. Homologous OsFAD3 is more active than GmFAD3-1 in catalysing LA conversion to ALA in rice seeds. Overexpression of ER-localized GmFAD3-2/3 and chloroplast-localized OsFAD7/8 had less effect on increasing the ALA content in rice seeds. The GluC promoter is advantageous compared with Ubi-1 in this experimental system. The enhanced ALA was preferentially located at the sn-2 position in triacylglycerols. A meal-size portion of high ALA rice would meet >80% of the daily adult ALA requirement. The ALA-rich rice could be expected to ameliorate much of the global dietary ALA deficiency.
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