ArticleJournal of clinical microbiology2012
Development of a novel genus-specific real-time PCR assay for detection and differentiation of Bartonella species and genotypes.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
99 citing papers in PubMed, 164 citations in OpenAlex.
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- Clinical insights into Candidatus Mycoplasma haemominutum-associated anaemia in naturally infected cats: flea-associated risk and paradoxical clinicopathological findings.Irish veterinary journal · 2026Article
- Ticks and fleas of the critically endangered mountain pygmy-possum (International journal for parasitology. Parasites and wildlife · 2026Article
- Genetic uniformity and occurrence ofParasite epidemiology and control · 2026Article
- Flea infestation and molecular detection of Rickettsia and Bartonella spp. in domestic dog-associated fleas from the inter-Andean basin of Cundinamarca, Colombia.Medical and veterinary entomology · 2026Article
- Investigation of neglected bacterial pathogens provides molecular and serological evidence of Bartonella spp. in patients with acute undifferentiated febrile illness in Villeta, Colombia.Parasites & vectors · 2026Article
- Hemotropic mycoplasmas and Bartonella spp. in dogs from Southern Italy: prevalence and risk factors.Veterinary research communications · 2026Article
- Community-engaged passive tick surveillance highlights the diversity of ticks and tick-borne pathogens in Illinois.PloS one · 2026Article
- Cell surface Toll-like receptor polymorphisms influenceiScience · 2025Article
- Small mammals as hosts of vector-borne pathogens in the High Tatra Mountains region in Slovakia, Central Europe.Current research in parasitology & vector-borne diseases · 2025Article
- Molecular Detection and Isolation ofTransboundary and emerging diseases · 2025Article
- Contrasting pathogen prevalence between tick and dog populations at Chornobyl.Parasites & vectors · 2024Article
- Blood culture-negative endocarditis caused by Bartonella quintana in Iran.Scientific reports · 2024Article
- Genetic diversity of Bartonella species in small mammals in the Qinghai Menyuan section of Qilian Mountain National Park, China.Scientific reports · 2024Article
- Prevalence and genetic diversity of rodent-associatedHeliyon · 2024Article
- Detection ofMicrobiology spectrum · 2024Article
- Epidemiological survey and genetic diversity of Bartonella in fleas collected from rodents in Fujian Province, Southeast China.Parasites & vectors · 2024Article
- Potential drivers of vector-borne pathogens in urban environments: European hedgehogs (One health (Amsterdam, Netherlands) · 2024Article
- Article
- Molecular Prevalence, Genetic Diversity, and Tissue Tropism ofAnimals : an open access journal from MDPI · 2024Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genus Bartonella includes numerous species with varied host associations, including several that infect humans. Development of a molecular diagnostic method capable of detecting the diverse repertoire of Bartonella species while maintaining genus specificity has been a challenge. We developed a novel real-time PCR assay targeting a 301-bp region of the ssrA gene of Bartonella and demonstrated specific amplification in over 30 Bartonella species, subspecies, and strains. Subsequent analysis of ssrA sequences was sufficient to discriminate Bartonella species and provided phylogenetic data consistent with that of gltA, a commonly used gene for differentiating Bartonella genotypes. Using this assay, we identified Bartonella DNA in 29% and 47% of blood specimens from elk in Wyoming and cattle in the Republic of Georgia, respectively. Sequence analysis of a subset of genotypes from elk specimens revealed a cluster most closely related to Bartonella capreoli, and genotypes from cattle were identified as Bartonella bovis, both Bartonella species commonly found in wild and domestic ruminants. Considering the widespread geographic distribution and infectivity potential to a variety of hosts, this assay may be an effective diagnostic method for identification of Bartonella infections in humans and have utility in Bartonella surveillance studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.