ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 244 papers.
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Who cites it
244 citing papers in PubMed, 374 citations in OpenAlex.
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- A Cre-dependent reporter mouse for quantitative real-time imaging of protein kinase A activity dynamics.Scientific reports · 2024Article
- β-adrenergic signaling triggers enteric glial reactivity and acute enteric gliosis during surgery.Journal of neuroinflammation · 2023Article
- A Cre-dependent reporter mouse for quantitative real-time imaging of Protein Kinase A activity dynamics.bioRxiv : the preprint server for biology · 2023Article
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- Excitatory and Inhibitory Descending Commissural Interneurons Differentially Integrate Supraspinal and Segmental Sensory Signals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Calcium imaging and analysis of the jugular-nodose ganglia enables identification of distinct vagal sensory neuron subsets.Journal of neural engineering · 2023Article
- Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity.Scientific reports · 2023Article
- Mesoscale calcium imagingFrontiers in neuroscience · 2023Review
184 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
Abstract
Fluorescent calcium indicator proteins, such as GCaMP3, allow imaging of activity in genetically defined neuronal populations. GCaMP3 can be expressed using various gene delivery methods, such as viral infection or electroporation. However, these methods are invasive and provide inhomogeneous and nonstationary expression. Here, we developed a genetic reporter mouse, Ai38, which expresses GCaMP3 in a Cre-dependent manner from the ROSA26 locus, driven by a strong CAG promoter. Crossing Ai38 with appropriate Cre mice produced robust GCaMP3 expression in defined cell populations in the retina, cortex, and cerebellum. In the primary visual cortex, visually evoked GCaMP3 signals showed normal orientation and direction selectivity. GCaMP3 signals were rapid, compared with virally expressed GCaMP3 and synthetic calcium indicators. In the retina, Ai38 allowed imaging spontaneous calcium waves in starburst amacrine cells during development, and light-evoked responses in ganglion cells in adult tissue. Our results show that the Ai38 reporter mouse provides a flexible method for targeted expression of GCaMP3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.