ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Measuring the firing rate of high-resistance neurons with cell-attached recording.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Functional interactions between anteroventral periventricular kisspeptin neurons and gonadotropin-releasing hormone neurons in female mice.Journal of neuroendocrinology · 2026Article
- Challenges and Insights in Patch-Clamp Studies: From Cell-Attached to Whole-Cell Configurations.Current issues in molecular biology · 2026Review
- Sex and Time of Day Alter the Interactions Between Hypothalamic Glia and the Neural Circuits Controlling Reproduction.Endocrinology · 2025Article
- A first morphological and electrophysiological characterization of Fañanas cells of the mouse cerebellum.The Journal of physiology · 2025Article
- Adaptive remodeling of rat adrenomedullary stimulus-secretion coupling in a chronic hypertensive environment.Cellular and molecular life sciences : CMLS · 2024Article
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- KCa1.1 channels contribute to optogenetically driven post-stimulation silencing in cerebellar molecular layer interneurons.The Journal of general physiology · 2023Article
- On the accuracy of cell-attached current-clamp recordings from cortical neurons.Frontiers in molecular neuroscience · 2022Article
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- Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses.Nature protocols · 2021Article
- A sodium background conductance controls the spiking pattern of mouse adrenal chromaffin cells in situ.The Journal of physiology · 2021Article
- Large, Stable Spikes Exhibit Differential Broadening in Excitatory and Inhibitory Neocortical Boutons.Cell reports · 2021Article
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- Ovarian Androgens Maintain High GnRH Neuron Firing Rate in Adult Prenatally-Androgenized Female Mice.Endocrinology · 2020Article
- Cellular and synaptic phenotypes lead to disrupted information processing in Fmr1-KO mouse layer 4 barrel cortex.Nature communications · 2019Article
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- Aberrant cerebellar Purkinje cell function repaired in vivo by fusion with infiltrating bone marrow-derived cells.Acta neuropathologica · 2018Article
- Glutamatergic Transmission to Hypothalamic Kisspeptin Neurons Is Differentially Regulated by Estradiol through Estrogen Receptor α in Adult Female Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Accelerated Development of the First-Order Central Auditory Neurons With Spontaneous Activity.Frontiers in molecular neuroscience · 2018Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-attached recording is extensively used to study the firing rate of mammalian neurons, but potential limitations of the method have not been investigated in detail. Here we perform cell-attached recording of molecular layer interneurons in cerebellar slices from rats and mice, and we study how experimental conditions influence the measured firing rate. We find that this rate depends on time in cell-attached mode, on pipette potential, and on pipette ionic composition. In the first minute after sealing, action currents are variable in shape and size, presumably reflecting membrane instability. The firing rate remains approximately constant during the first 4 min after sealing and gradually increases afterward. Making the pipette potential more positive leads to an increase in the firing rate, with a steeper dependence on voltage if the pipette solution contains K(+) as the main cation than if it contains Na(+). Ca(2+) imaging experiments show that establishing a cell-attached recording can result in an increased somatic Ca(2+) concentration, reflecting an increased firing rate linked to an increase in the pipette-cell conductance. Pipette effects on cell firing are traced to a combination of passive electrical coupling, opening of voltage- and Ca(2+)-sensitive K(+) channels (BK channels) after action potentials, and random activation of voltage-insensitive, presumably mechanosensitive, cationic channels. We conclude that, unless experimental conditions are optimized, cell-attached recordings in small neurons may report erroneous firing rates.
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