ArticleThe Journal of biological chemistry2012
Fast modulation of μ-opioid receptor (MOR) recycling is mediated by receptor agonists.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Differential regulation of G protein-coupled receptor-associated proteins in the caudate and the putamen of cynomolgus macaques following chronic ethanol drinking.Journal of neurochemistry · 2024Article
- A Balancing Act: Learning from the Past to Build a Future-Focused Opioid Strategy.Annual review of physiology · 2024Review
- Ketamine Improves Desensitization of µ-Opioid Receptors Induced by Repeated Treatment with Fentanyl but Not with Morphine.Biomolecules · 2022Article
- Homologous Regulation of Mu Opioid Receptor Recycling by GMolecular pharmacology · 2019Article
- Spatial encoding of GPCR signaling in the nervous system.Current opinion in cell biology · 2019Review
- Retromer stops beta-arrestin 1-mediated signaling from internalized cannabinoid 2 receptors.Molecular biology of the cell · 2017Article
- Mechanisms of Biased β-Arrestin-Mediated Signaling Downstream from the Cannabinoid 1 Receptor.Molecular pharmacology · 2016Article
- Imaging GPCRs trafficking and signaling with total internal reflection fluorescence microscopy in cultured neurons.Methods in cell biology · 2016Article
- Agonist Binding and Desensitization of the μ-Opioid Receptor Is Modulated by Phosphorylation of the C-Terminal Tail Domain.Molecular pharmacology · 2015Article
- Cell-autonomous regulation of Mu-opioid receptor recycling by substance P.Cell reports · 2015Article
- Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Recent advances on the δ opioid receptor: from trafficking to function.British journal of pharmacology · 2015Review
- Postendocytic Sorting of Adrenergic and Opioid Receptors: New Mechanisms and Functions.Progress in molecular biology and translational science · 2015Review
- Endothelin-converting enzyme 2 differentially regulates opioid receptor activity.British journal of pharmacology · 2015Article
- Ligand-specific endocytic dwell times control functional selectivity of the cannabinoid receptor 1.Nature communications · 2014Article
- Morphine-induced trafficking of a mu-opioid receptor interacting protein in rat locus coeruleus neurons.Progress in neuro-psychopharmacology & biological psychiatry · 2014Article
- Rab5 and Rab4 regulate axon elongation in the Xenopus visual system.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Imaging of kiss-and-run exocytosis of surface receptors in neuronal cultures.Frontiers in cellular neuroscience · 2014Article
- SNARE proteins are essential in the potentiation of NMDA receptors by group II metabotropic glutamate receptors.The Journal of physiology · 2013Article
- Crucial involvement of actin filaments in celecoxib and morphine analgesia in a model of inflammatory pain.Journal of pain research · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The μ-opioid receptor (MOR) is a member of the G protein-coupled receptor family and the main target of endogenous opioid neuropeptides and morphine. Upon activation by ligands, MORs are rapidly internalized via clathrin-coated pits in heterologous cells and dissociated striatal neurons. After initial endocytosis, resensitized receptors recycle back to the cell surface by vesicular delivery for subsequent cycles of activation. MOR trafficking has been linked to opioid tolerance after acute exposure to agonist, but it is also involved in the resensitization process. Several studies describe the regulation and mechanism of MOR endocytosis, but little is known about the recycling of resensitized receptors to the cell surface. To study this process, we induced internalization of MOR with [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-enkephalin (DAMGO) and morphine and imaged in real time single vesicles recycling receptors to the cell surface. We determined single vesicle recycling kinetics and the number of receptors contained in them. Then we demonstrated that rapid vesicular delivery of recycling MORs to the cell surface was mediated by the actin-microtubule cytoskeleton. Recycling was also dependent on Rab4, Rab11, and the Ca(2+)-sensitive motor protein myosin Vb. Finally, we showed that recycling is acutely modulated by the presence of agonists and the levels of cAMP. Our work identifies a novel trafficking mechanism that increases the number of cell surface MORs during acute agonist exposure, effectively reducing the development of opioid tolerance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.