ArticleThe Journal of biological chemistry2012
L-galactono-1,4-lactone dehydrogenase (GLDH) forms part of three subcomplexes of mitochondrial complex I in Arabidopsis thaliana.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 98 citations in OpenAlex.
- PCIS1 is an essential factor in mitochondrial RNA splicing and complex I biogenesis, with distinct effects in null and downregulated mutants.Plant cell reports · 2026Article
- Comprehensive evaluation of compound saline-alkali tolerance and gene mining by GWAS in Vicia faba L.BMC plant biology · 2025Article
- Application of 4-CPA or ethanol enhances plant growth and fruit quality of phyA mutant under heat stress.Scientific reports · 2025Article
- The complex I subunit B22 contains a LYR domain that is crucial for an interaction with the mitochondrial acyl carrier protein SDAP1.The Plant journal : for cell and molecular biology · 2025Article
- Structure, mechanism, and evolution of the last step in vitamin C biosynthesis.Nature communications · 2024Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Using cryo-EM to understand the assembly pathway of respiratory complex I.Acta crystallographica. Section D, Structural biology · 2024Article
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis.Antioxidants (Basel, Switzerland) · 2023Review
- The conditional mitochondrial protein complexome in the Arabidopsis thaliana root and shoot.Plant communications · 2023Article
- Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis.Plant physiology · 2023Article
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Proteolytic regulation of mitochondrial oxidative phosphorylation components in plants.Biochemical Society transactions · 2022Review
- Maturation and Assembly of Iron-Sulfur Cluster-Containing Subunits in the Mitochondrial Complex I From Plants.Frontiers in plant science · 2022Review
- A ferredoxin bridge connects the two arms of plant mitochondrial complex I.The Plant cell · 2021Article
- Elevating Ascorbate in Arabidopsis Stimulates the Production of Abscisic Acid, Phaseic Acid, and to a Lesser Extent Auxin (IAA) and Jasmonates, Resulting in Increased Expression ofInternational journal of molecular sciences · 2021Article
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- Stress signalling dynamics of the mitochondrial electron transport chain and oxidative phosphorylation system in higher plants.Annals of botany · 2020Review
- Article
- A Mitochondrial LYR Protein Is Required for Complex I Assembly.Plant physiology · 2019Article
- Vitamin C in Plants: From Functions to Biofortification.Antioxidants (Basel, Switzerland) · 2019Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-galactono-1,4-lactone dehydrogenase (GLDH) catalyzes the terminal step of the Smirnoff-Wheeler pathway for vitamin C (l-ascorbate) biosynthesis in plants. A GLDH in gel activity assay was developed to biochemically investigate GLDH localization in plant mitochondria. It previously has been shown that GLDH forms part of an 850-kDa complex that represents a minor form of the respiratory NADH dehydrogenase complex (complex I). Because accumulation of complex I is disturbed in the absence of GLDH, a role of this enzyme in complex I assembly has been proposed. Here we report that GLDH is associated with two further protein complexes. Using native gel electrophoresis procedures in combination with the in gel GLDH activity assay and immunoblotting, two mitochondrial complexes of 470 and 420 kDa were identified. Both complexes are of very low abundance. Protein identifications by mass spectrometry revealed that they include subunits of complex I. Finally, the 850-kDa complex was further investigated and shown to include the complete "peripheral arm" of complex I. GLDH is attached to a membrane domain, which represents a major fragment of the "membrane arm" of complex I. Taken together, our data further support a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates.
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