ArticleThe Journal of biological chemistry2012
PpsR, a regulator of heme and bacteriochlorophyll biosynthesis, is a heme-sensing protein.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- The blue light-dependent LOV-protein LdaP ofFrontiers in microbiology · 2024Article
- Origin and functional diversification of PAS domain, a ubiquitous intracellular sensor.Science advances · 2023Article
- Molecular insights into the role of heme in the transcriptional regulatory system AppA/PpsR.Biophysical journal · 2022Article
- Article
- Heme-dependent Inactivation of 5-Aminolevulinate Synthase from Caulobacter crescentus.Scientific reports · 2018Article
- SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall inFrontiers in cellular and infection microbiology · 2016Article
- Members of the PpaA/AerR Antirepressor Family Bind Cobalamin.Journal of bacteriology · 2015Article
- Redox-dependent Ligand Switching in a Sensory Heme-binding GAF Domain of the Cyanobacterium Nostoc sp. PCC7120.The Journal of biological chemistry · 2015Article
- Article
- The extracellular heme-binding protein HbpS from the soil bacterium Streptomyces reticuli is an aquo-cobalamin binder.The Journal of biological chemistry · 2014Article
- Multi-PAS domain-mediated protein oligomerization of PpsR from Rhodobacter sphaeroides.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Vitamin B12 regulates photosystem gene expression via the CrtJ antirepressor AerR in Rhodobacter capsulatus.Molecular microbiology · 2014Article
- Article
- Controlling the delicate balance of tetrapyrrole biosynthesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.Nature structural & molecular biology · 2013Article
- Article
- Heme sensor proteins.The Journal of biological chemistry · 2013Review
- A model for tetrapyrrole synthesis as the primary mechanism for plastid-to-nucleus signaling during chloroplast biogenesis.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heme-mediated regulation, presented in many biological processes, is achieved in part with proteins containing heme regulatory motif. In this study, we demonstrate that FLAG-tagged PpsR isolated from Rhodobacter sphaeroides cells contains bound heme. In vitro heme binding studies with tagless apo-PpsR show that PpsR binds heme at a near one-to-one ratio with a micromolar binding constant. Mutational and spectral assays suggest that both the second Per-Arnt-Sim (PAS) and DNA binding domains of PpsR are involved in the heme binding. Furthermore, we show that heme changes the DNA binding patterns of PpsR and induces different responses of photosystem genes expression. Thus, PpsR functions as both a redox and heme sensor to coordinate the amount of heme, bacteriochlorophyll, and photosystem apoprotein synthesis thereby providing fine tune control to avoid excess free tetrapyrrole accumulation.
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Registered trials
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