Evidence map›Paper›PMID 22378731›Full record

ArticleThe American journal of clinical nutrition2012

Association of PLA2G4A with myocardial infarction is modulated by dietary PUFAs.

Jaana Hartiala, Elizabeth Gilliam, Susanna Vikman, Hannia Campos, Hooman Allayee

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of clinical nutrition, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 2 pooled it
1.0field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 2 syntheses or guidelines pooled it, 18 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Effects of APOE4 on omega-3 brain metabolism across the lifespan.Trends in endocrinology and metabolism: TEM · 2024
    Review
  5. The Link between Prostanoids and Cardiovascular Diseases.International journal of molecular sciences · 2023
    Review
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jaana HartialaDepartment of Preventive Medicine, the University of Southern California Keck School of Medicine, Los Angeles, CA, USA.
Elizabeth Gilliam
Susanna Vikman
Hannia Campos
Hooman Allayee
University of Southern California · US

Funding

GENE-DIET INTERACTIONS AND HEART DISEASER01HL060692 · NHLBI · HARVARD UNIVERSITY (SCH OF PUBLIC HLTH) · PI CAMPOS, HANNIA · 1999 to 2003
$4.1M
Role of the 5-Lipoxygenase Pathway in AtherosclerosisR01HL079353 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI ALLAYEE, HOOMAN · 2005 to 2009
$2.8M
EXTRAMURAL RESEARCH FACILITIES CONTRUCTION PROJECTC06CA062528 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KEDES, LAURENCE H · 1993 to 1994
–
EXTRAMURAL RESEARCH FACILITIES CONSTRUCTIONC06RR010600 · NCRR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KEDES, LAURENCE H · 1994 to 1994
–
RESEARCH FACILITIES IMPROVEMENT PROGRAM AND REMODELINGC06RR014514 · NCRR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KEDES, LAURENCE H · 1999 to 1999
–
NCI NIH HHS C06 CA62528-01NCRR NIH HHS C06 RR10600-01NCRR NIH HHS C06 RR14514-01NHLBI NIH HHS HL060692NHLBI NIH HHS HL079353NHLBI NIH HHS R01 HL060692NHLBI NIH HHS R01 HL079353
6 · The paper itself

Abstract

backgroundLeukotrienes are proinflammatory molecules derived from dietary PUFAs and have been associated with cardiovascular disease (CVD). We previously reported that an A→G variant (rs12746200) of the cytosolic phospholipase A2 group IVA gene (PLA2G4A), which encodes the enzyme that liberates PUFAs from cellular membranes for leukotriene synthesis, decreases the risk of CVD.

objectiveWe sought to replicate these initial observations with a more clinically relevant phenotype, such as myocardial infarction (MI), and to determine whether dietary PUFAs mediate this association.

designIn a Costa Rican case-control data set (n = 3971), rs12746200 was genotyped and was tested for an association with MI. Functional experiments were carried out to determine whether rs12746200 led to differences in mRNA expression.

resultsRisk of MI was significantly lower in AG/GG subjects than in AA homozygotes (OR: 0.86; 95% CI: 0.75, 0.99; P = 0.040). The reduced risk of MI was observed primarily in AG/GG subjects who were above the median for intake of dietary omega-6 (n-6) PUFAs (OR: 0.71; 95% CI: 0.59, 0.87; P-interaction = 0.005). A similar analysis with dietary omega-3 (n-3) PUFAs did not show a statistically significant nutrigenetic association (P-interaction = 0.23). Functional analysis in human aortic endothelial cells showed that the carriers of the G allele had significantly lower PLA2G4A gene expression (P = 0.014), consistent with the atheroprotective association of this variant.

conclusionThese results replicate the association of rs12746200 with CVD phenotypes and provide evidence that the protective association of this functional PLA2G4A variant is mediated by dietary PUFAs.

Indexed as

DietPolymorphism, Single NucleotideAgedAortaCase-Control StudiesCells, CulturedCosta RicaEndothelium, VascularFatty Acids, Omega-6FemaleGene Expression Regulation, EnzymologicGenetic Association StudiesGroup IV Phospholipases A2HumansMaleMiddle AgedFatty Acids, Omega-6Group IV Phospholipases A2PLA2G4A protein, humanRNA, Messenger

Identifiers

PMID22378731
PMCPMC3302367
OpenAlexW2151043410

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.