ArticleGenetics2012
A naturally thermolabile activity compromises genetic analysis of telomere function in Saccharomyces cerevisiae.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Widely spaced and divergent inverted repeats become a potent source of chromosomal rearrangements in long single-stranded DNA regions.Nucleic acids research · 2023Article
- Investigating the role of G-quadruplexes atMicrobial cell (Graz, Austria) · 2022Article
- The Bur1 cyclin-dependent kinase regulates telomere length in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2022Article
- Article
- Saccharomyces cerevisiae Mus81-Mms4 prevents accelerated senescence in telomerase-deficient cells.PLoS genetics · 2020Article
- Loss of Cdc13 causes genome instability by a deficiency in replication-dependent telomere capping.PLoS genetics · 2020Article
- Adaptation in replicative senescence: a risky business.Current genetics · 2019Review
- Adaptation to DNA damage checkpoint in senescent telomerase-negative cells promotes genome instability.Genes & development · 2018Article
- Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The global role for Cdc13 and Yku70 in preventing telomere resection across the genome.DNA repair · 2018Article
- Using Separation-of-Function Mutagenesis To Define the Full Spectrum of Activities Performed by the Est1 Telomerase SubunitGenetics · 2018Article
- A Slowed Cell Cycle Stabilizes the Budding Yeast Genome.Genetics · 2017Article
- Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt.Nucleic acids research · 2016Article
- Candida albicans repetitive elements display epigenetic diversity and plasticity.Scientific reports · 2016Article
- Cell populations can use aneuploidy to survive telomerase insufficiency.Nature communications · 2015Article
- Telomerase RNA stem terminus element affects template boundary element function, telomere sequence, and shelterin binding.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.Genetics · 2014Article
- Interplay between nonsense-mediated mRNA decay and DNA damage response pathways reveals that Stn1 and Ten1 are the key CST telomere-cap components.Cell reports · 2014Article
- The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1.Nucleic acids research · 2014Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The core assumption driving the use of conditional loss-of-function reagents such as temperature-sensitive mutations is that the resulting phenotype(s) are solely due to depletion of the mutant protein under nonpermissive conditions. However, prior published data, combined with observations presented here, challenge the generality of this assumption at least for telomere biology: for both wild-type yeast and strains bearing null mutations in telomere protein complexes, there is an additional phenotypic consequence when cells are grown above 34°. We propose that this synthetic phenotype is due to a naturally thermolabile activity that confers a telomere-specific defect, which we call the Tmp(-) phenotype. This prompted a re-examination of commonly used cdc13-ts and stn1-ts mutations, which indicates that these alleles are instead hypomorphic mutations that behave as apparent temperature-sensitive mutations due to the additive effects of the Tmp(-) phenotype. We therefore generated new cdc13-ts reagents, which are nonpermissive below 34°, to allow examination of cdc13-depleted phenotypes in the absence of this temperature-dependent defect. A return-to-viability experiment following prolonged incubation at 32°, 34°, and 36° with one of these new cdc13-ts alleles argues that the accelerated inviability previously observed at 36° in cdc13-1 rad9-Δ mutant strains is a consequence of the Tmp(-) phenotype. Although this study focused on telomere biology, viable null mutations that confer inviability at 36° have been identified for multiple cellular pathways. Thus, phenotypic analysis of other aspects of yeast biology may similarly be compromised at high temperatures by pathway-specific versions of the Tmp(-) phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.