ArticleBMC microbiology2012
Tandem repeat markers as novel diagnostic tools for high resolution fingerprinting of Wolbachia.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 75 citations in OpenAlex.
- Historic Museum Samples Provide Evidence for a Recent Replacement of Wolbachia Types in European Drosophila melanogaster.Molecular biology and evolution · 2023Article
- Wolbachia has subtle effects on thermal preference in highly inbred Drosophila melanogaster which vary with life stage and environmental conditions.Scientific reports · 2023Article
- Interaction of High Temperature Stress andInsects · 2023Article
- Widespread phages of endosymbionts: Phage WO genomics and the proposed taxonomic classification of Symbioviridae.PLoS genetics · 2022Article
- Complex effects of environment and Wolbachia infections on the life history of Drosophila melanogaster hosts.Journal of evolutionary biology · 2022Article
- Restriction ofmBio · 2022Article
- Parallel Sequencing of Wolbachia wCer2 from Donor and Novel Hosts Reveals Multiple Incompatibility Factors and Genome Stability after Host Transfers.Genome biology and evolution · 2020Article
- Review
- The Wolbachia mobilome in Culex pipiens includes a putative plasmid.Nature communications · 2019Article
- Wolbachia modifies thermal preference in Drosophila melanogaster.Environmental microbiology · 2018Article
- Insertion sequence polymorphism and genomic rearrangements uncover hidden Wolbachia diversity in Drosophila suzukii and D. subpulchrella.Scientific reports · 2017Article
- Macronutrients mediate the functional relationship between Drosophila and Wolbachia.Proceedings. Biological sciences · 2015Article
- Mutualism breakdown by amplification of Wolbachia genes.PLoS biology · 2015Article
- Article
- A new multiple-locus variable-number tandem repeat analysis reveals different clusters for Anaplasma phagocytophilum circulating in domestic and wild ruminants.Parasites & vectors · 2014Article
- More than fishing in the dark: PCR of a dispersed sequence produces simple but ultrasensitive Wolbachia detection.BMC microbiology · 2014Article
- Deep conservation of human protein tandem repeats within the eukaryotes.Molecular biology and evolution · 2014Article
- Microsatellite and Wolbachia analysis in Rhagoletis cerasi natural populations: population structuring and multiple infections.Ecology and evolution · 2014Article
- Global Wolbachia prevalence, titer fluctuations and their potential of causing cytoplasmic incompatibilities in tsetse flies and hybrids of Glossina morsitans subgroup species.Journal of invertebrate pathology · 2013Article
- Wolbachia variants induce differential protection to viruses in Drosophila melanogaster: a phenotypic and phylogenomic analysis.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundStrains of the endosymbiotic bacterium Wolbachia pipientis are extremely diverse both genotypically and in terms of their induced phenotypes in invertebrate hosts. Despite extensive molecular characterisation of Wolbachia diversity, little is known about the actual genomic diversity within or between closely related strains that group tightly on the basis of existing gene marker systems, including Multiple Locus Sequence Typing (MLST). There is an urgent need for higher resolution fingerprinting markers of Wolbachia for studies of population genetics, horizontal transmission and experimental evolution.
resultsThe genome of the wMel Wolbachia strain that infects Drosophila melanogaster contains inter- and intragenic tandem repeats that may evolve through expansion or contraction. We identified hypervariable regions in wMel, including intergenic Variable Number Tandem Repeats (VNTRs), and genes encoding ankyrin (ANK) repeat domains. We amplified these markers from 14 related Wolbachia strains belonging to supergroup A and were successful in differentiating size polymorphic alleles. Because of their tandemly repeated structure and length polymorphism, the markers can be used in a PCR-diagnostic multilocus typing approach, analogous to the Multiple Locus VNTR Analysis (MLVA) established for many other bacteria and organisms. The isolated markers are highly specific for supergroup A and not informative for other supergroups. However, in silico analysis of completed genomes from other supergroups revealed the presence of tandem repeats that are variable and could therefore be useful for typing target strains.
conclusionsWolbachia genomes contain inter- and intragenic tandem repeats that evolve through expansion or contraction. A selection of polymorphic tandem repeats is a novel and useful PCR diagnostic extension to the existing MLST typing system of Wolbachia, as it allows rapid and inexpensive high-throughput fingerprinting of closely related strains for which polymorphic markers were previously lacking.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.