ArticleThe Journal of biological chemistry2012
Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 63 citations in OpenAlex.
- Ecdysone signaling acts as a regulator of immunity and microbiota balance, shapingFrontiers in immunology · 2026Article
- Azadirachtin disrupts ecdysone signaling and alters sand fly immunity.Parasites & vectors · 2024Article
- Immune Reactions of Vector Insects to Parasites and Pathogens.Microorganisms · 2024Review
- Genomic analysis of two phlebotomine sand fly vectors of Leishmania from the New and Old World.PLoS neglected tropical diseases · 2023Article
- Phenotypical Differences betweenPathogens (Basel, Switzerland) · 2023Article
- Evidence of a conserved mammalian immunosuppression mechanism inFrontiers in immunology · 2023Article
- RNAi-mediated gene silencing ofFrontiers in physiology · 2023Article
- Host-Parasite Interactions: Regulation of Leishmania Infection in Sand Fly.Acta parasitologica · 2022Review
- Article
- Gut Microbiota Dynamics in Natural Populations ofMicroorganisms · 2021Article
- The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal.Parasites & vectors · 2021Article
- Article
- Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence inmSphere · 2020Article
- Leishmania infection induces a limited differential gene expression in the sand fly midgut.BMC genomics · 2020Article
- Article
- Functional genomics in sand fly-derived Leishmania promastigotes.PLoS neglected tropical diseases · 2019Review
- Wolbachia introduction into Lutzomyia longipalpis (Diptera: Psychodidae) cell lines and its effects on immune-related gene expression and interaction with Leishmania infantum.Parasites & vectors · 2019Article
- Article
- Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9.PLoS neglected tropical diseases · 2018Article
- Leishmania, microbiota and sand fly immunity.Parasitology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Female phlebotomine sand flies Lutzomyia longipalpis naturally harbor populations of the medically important Leishmania infantum (syn. Leishmania chagasi) parasite in the gut, but the extent to which the parasite interacts with the immune system of the insect vector is unknown. To investigate the sand fly immune response and its interaction with the Leishmania parasite, we identified a homologue for caspar, a negative regulator of immune deficiency signaling pathway. We found that feeding antibiotics to adult female L. longipalpis resulted in an up-regulation of caspar expression relative to controls. caspar was differentially expressed when females were fed on gram-negative and gram-positive bacterial species. caspar expression was significantly down-regulated in females between 3 and 6 days after a blood feed containing Leishmania mexicana amastigotes. RNA interference was used to deplete caspar expression in female L. longipalpis, which were subsequently fed with Leishmania in a blood meal. Sand fly gut populations of both L. mexicana and L. infantum were significantly reduced in caspar-depleted females. The prevalence of L. infantum infection in the females fell from 85 to 45%. Our results provide the first insight into the operation of immune homeostasis in phlebotomine sand flies during the growth of bacterial and Leishmania populations in the digestive tract. We have demonstrated that the activation of the sand fly immune system, via depletion of a single gene, can lead to the abortion of Leishmania development and the disruption of transmission by the phlebotomine sand fly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.