ArticleThe Journal of biological chemistry2012
MarR-type transcriptional regulator ChlR activates expression of tetrapyrrole biosynthesis genes in response to low-oxygen conditions in cyanobacteria.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- From photosynthetic electron flow to gene regulation: redox signal transduction in cyanobacteria.FEMS microbiology reviews · 2026Review
- Black Sea picocyanobacteria exploit ancestral traits for energy and chlorophyll production in dark anoxia.ISME communications · 2026Article
- PicR as a MarR Family Transcriptional Repressor Multiply Controls the Transcription of Picolinic Acid Degradation Gene ClusterApplied and environmental microbiology · 2022Article
- The cyanobacteriumProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Evolution of Ycf54-independent chlorophyll biosynthesis in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism.Scientific reports · 2018Article
- The Complex Transcriptional Response ofG3 (Bethesda, Md.) · 2017Article
- Comparison of the terrestrial cyanobacterium Leptolyngbya sp. NIES-2104 and the freshwater Leptolyngbya boryana PCC 6306 genomes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- Evolutionary Aspects and Regulation of Tetrapyrrole Biosynthesis in Cyanobacteria under Aerobic and Anaerobic Environments.Life (Basel, Switzerland) · 2015Review
- Interdependence of tetrapyrrole metabolism, the generation of oxidative stress and the mitigative oxidative stress response.Redox biology · 2015Review
- ChlR protein of Synechococcus sp. PCC 7002 is a transcription activator that uses an oxygen-sensitive [4Fe-4S] cluster to control genes involved in pigment biosynthesis.The Journal of biological chemistry · 2014Article
- Comparative genome analysis of the closely related Synechocystis strains PCC 6714 and PCC 6803.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Transcriptional regulators ChlR and CnfR are essential for diazotrophic growth in nonheterocystous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structural mechanism of transcription regulation of the Staphylococcus aureus multidrug efflux operon mepRA by the MarR family repressor MepR.Nucleic acids research · 2014Article
- A novel "oxygen-induced" greening process in a cyanobacterial mutant lacking the transcriptional activator ChlR involved in low-oxygen adaptation of tetrapyrrole biosynthesis.The Journal of biological chemistry · 2014Article
- The chlorite dismutase (HemQ) from Staphylococcus aureus has a redox-sensitive heme and is associated with the small colony variant phenotype.The Journal of biological chemistry · 2013Article
- Oxidation-sensing regulator AbfR regulates oxidative stress responses, bacterial aggregation, and biofilm formation in Staphylococcus epidermidis.The Journal of biological chemistry · 2013Article
- Microevolution in cyanobacteria: re-sequencing a motile substrain of Synechocystis sp. PCC 6803.DNA research : an international journal for rapid publication of reports on genes and genomes · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxygen is required for three enzyme reactions in chlorophyll and bilin biosynthesis pathways: coproporphyrinogen III oxidase (HemF), heme oxygenase (HO1), and Mg-protoporphyrin IX monomethylester cyclase (ChlA(I)). The cyanobacterium Synechocystis sp. PCC 6803 has alternative enzymes, HemN, HO2, and ChlA(II), to supply chlorophyll/bilins even under low-oxygen environments. The three genes form an operon, chlA(II)-ho2-hemN, that is induced in response to low-oxygen conditions to bypass the oxygen-dependent reactions. Here we identified a transcriptional regulator for the induction of the operon in response to low-oxygen conditions. A pseudorevertant, Δho1R, was isolated from a HO1-lacking mutant Δho1 that is lethal under aerobic conditions. Δho1R grew well even under aerobic conditions. In Δho1R, HO2 that is induced only under low-oxygen conditions was anomalously expressed under aerobic conditions to complement the loss of HO1. A G-to-C transversion in sll1512 causing the amino acid change from aspartate 35 to histidine was identified as the relevant mutation by resequencing of the Δho1R genome. Sll1512 is a MarR-type transcriptional regulator. An sll1512-lacking mutant grew poorly under low-oxygen conditions with a remarked decrease in Chl content that would be caused by the suppressed induction of the chlA(II) and hemN genes in Chl biosynthesis under low-oxygen conditions. These results demonstrated that Sll1512 is an activator in response to low-oxygen environments and that the D35H variant becomes a constitutive activator. This hypothesis was supported by a gel shift assay showing that the Sll1512-D35H variant binds to the DNA fragment upstream of the operon. We propose to name sll1512 chlR.
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