ArticleThe Laryngoscope2012
Differences in neuromuscular junctions of laryngeal and limb muscles in rats.
Article in The Laryngoscope, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- The role of ultrasonic vocalizations in rat laryngological investigations.Physiology & behavior · 2025Review
- Differences in neuromuscular junctions between intrinsic muscles of the forepaw and biceps muscles in rats.Histology and histopathology · 2024Article
- Thyroarytenoid Oxidative Metabolism and Synaptic Signaling Dysregulation in the Female Pink1-/- Rat.The Laryngoscope · 2023Article
- NMJ-related diseases beyond the congenital myasthenic syndromes.Frontiers in cell and developmental biology · 2023Review
- The Effects of Menopause on Neuromuscular Parameters of the Rat Vocal Folds.The Laryngoscope · 2021Article
- Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization.Brain sciences · 2021Review
- Stability of motor endplates is greater in the biceps than in the interossei in a rat model of obstetric brachial plexus palsy.Neural regeneration research · 2020Article
- Preparation of the Rat Vocal Fold for Neuromuscular Analyses.Journal of visualized experiments : JoVE · 2020Article
- A Tutorial of the Effects of Sex Hormones on Laryngeal Senescence and Neuromuscular Response to Exercise.Journal of speech, language, and hearing research : JSLHR · 2019Review
- Sexual dimorphism in laryngeal muscle fibers and ultrasonic vocalizations in the adult rat.The Laryngoscope · 2017Article
- Muscle fiber types composition and type identified endplate morphology of forepaw intrinsic muscles in the rat.Journal of muscle research and cell motility · 2016Article
- Article
- Expression of calcium-buffering proteins in rat intrinsic laryngeal muscles.Physiological reports · 2015Article
- Expression of atrophy-related transcription factors in the process of intrinsic laryngeal muscle atrophy after denervation.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2015Article
- Changes in Rat 50-kHz Ultrasonic Vocalizations During Dopamine Denervation and Aging: Relevance to Neurodegeneration.Current neuropharmacology · 2015Review
- The posterior cricoarytenoid muscle is spared from MuRF1-mediated muscle atrophy in mice with acute lung injury.PloS one · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
objectives/hypothesisLaryngeal muscles are specialized for fine control of voice, speech, and swallowing, and may differ from limb muscles in many aspects. Because muscles and their controlling motor neurons communicate via neuromuscular junctions (NMJs), we hypothesized that NMJs in laryngeal muscles have specialized characteristics different from limb muscles. STUDY
designIn vivo study.
methodsSingle muscle fibers from 12 Sprague-Dawley rats (six male, six female) were used to analyze the postsynaptic side of NMJs from laryngeal thyroarytenoid (TA), cricothyroid (CT), posterior cricoarytenoid (PCA), limb soleus (SOL), and extensor digitorum longus (EDL) muscles. NMJs were labeled with rhodamine-conjugated α-bungarotoxin. With confocal microscopy, we counted cluster fragments and measured the NMJ area, both absolute and normalized (corrected by muscle fiber diameter), for at least 10 single fibers from each muscle of each animal. Differences between genders were also compared.
resultsCluster fragments of postsynaptic NMJs were more numerous in PCA and TA compared to CT, SOL, and EDL muscles (P < .01) in both male and female rats. NMJ cluster fragments were more numerous in female than in male rats only in the TA muscle (P < .01). The absolute area covered by the NMJs showed SOL > EDL > PCA > CT > TA (P < .01); however, with normalization the SOL = EDL = PCA > CT = TA.
conclusionsDifferences found in NMJ surface and organization between laryngeal and limb muscle fibers may relate to specialized laryngeal muscle functions. Differences in NMJs between male and female rats were found only in the TA muscle, suggesting an underlying mechanism for some gender-specific laryngeal disorders related to abnormal TA muscle activity.
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