ArticleThe Plant cell2012
Transcriptome sequencing identifies SPL7-regulated copper acquisition genes FRO4/FRO5 and the copper dependence of iron homeostasis in Arabidopsis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 154 papers.
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Who cites it
154 citing papers in PubMed, 331 citations in OpenAlex.
- Comparative Transcriptomic Analysis Reveals Conserved and Nutrient-Specific Responses to Nutrient Deficiencies in Quinoa.Plants (Basel, Switzerland) · 2026Article
- The SVP-FRO6 Module Integrates the Control of Leaf Senescence and Flowering in Arabidopsis.Plant, cell & environment · 2026Article
- They look like gold: CITF1 and FIT interaction mediate cross-talk between iron and copper.The Plant cell · 2026Article
- Mineral nutrients as regulators of plant flowering time: A molecular perspective.Journal of integrative plant biology · 2026Review
- CITF1 interacts with FIT and regulates copper-iron crosstalk in Arabidopsis.The Plant cell · 2026Article
- The SPL-family transcription factor MpSPL3 orchestrates the proper regulation of vegetative and reproductive programs in Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2026Article
- Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner.Journal of experimental botany · 2025Article
- Age-dependent differential iron deficiency responses of rosette leaves during reproductive stages in Arabidopsis thaliana.Journal of experimental botany · 2025Article
- Molecular Evolution of Cu Transporters and Transcription Factors in Plant Response to Copper Stress.Plants (Basel, Switzerland) · 2025Review
- What Is Similar, What Is Different? Characterization of Mitoferrin-like Proteins fromInternational journal of molecular sciences · 2025Article
- Four ZIPs contribute to Zn, Fe, Cu and Mn acquisition at the outer root domain.PLoS genetics · 2025Article
- The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025Review
- Combating plant diseases through transition metal allocation.The New phytologist · 2025Review
- Barriers and carriers for transition metal homeostasis in plants.Plant communications · 2025Review
- Shedding light on iron nutrition: exploring intersections of transcription factor cascades in light and iron deficiency signaling.Journal of experimental botany · 2025Review
- Copper connections: coordinating transport, sensing and systemic signalling in plants.Quantitative plant biology · 2025Review
- The Transcription Factor VvbHLH053 Regulates the Expression of Copper Homeostasis-Associated GenesInternational journal of molecular sciences · 2024Article
- The landscape of fusion transcripts in plants: a new insight into genome complexity.BMC plant biology · 2024Article
- Diversification and conservation of DNA binding specificities of SPL family of transcription factors.bioRxiv : the preprint server for biology · 2024Article
- Micronutrient deficiency-induced oxidative stress in plants.Plant cell reports · 2024Review
94 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
The transition metal copper (Cu) is essential for all living organisms but is toxic when present in excess. To identify Cu deficiency responses comprehensively, we conducted genome-wide sequencing-based transcript profiling of Arabidopsis thaliana wild-type plants and of a mutant defective in the gene encoding SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), which acts as a transcriptional regulator of Cu deficiency responses. In response to Cu deficiency, FERRIC REDUCTASE OXIDASE5 (FRO5) and FRO4 transcript levels increased strongly, in an SPL7-dependent manner. Biochemical assays and confocal imaging of a Cu-specific fluorophore showed that high-affinity root Cu uptake requires prior FRO5/FRO4-dependent Cu(II)-specific reduction to Cu(I) and SPL7 function. Plant iron (Fe) deficiency markers were activated in Cu-deficient media, in which reduced growth of the spl7 mutant was partially rescued by Fe supplementation. Cultivation in Cu-deficient media caused a defect in root-to-shoot Fe translocation, which was exacerbated in spl7 and associated with a lack of ferroxidase activity. This is consistent with a possible role for a multicopper oxidase in Arabidopsis Fe homeostasis, as previously described in yeast, humans, and green algae. These insights into root Cu uptake and the interaction between Cu and Fe homeostasis will advance plant nutrition, crop breeding, and biogeochemical research.
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