ArticleNucleic acids research2012
Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
88 citing papers in PubMed.
- Overcoming the challenges of genome-editing essential genes.STAR protocols · 2026Review
- Multi-targeting zinc finger nuclease vector unsilences paternal UBE3A in a mouse model of Angelman syndrome.Gene therapy · 2026Article
- Advancing gene editing therapeutics: Clinical trials and innovative delivery systems across diverse diseases.Molecular therapy. Nucleic acids · 2025Review
- Strand-selective base editing of human mitochondrial DNA using mitoBEs.Nature biotechnology · 2024Article
- Compact zinc finger architecture utilizing toxin-derived cytidine deaminases for highly efficient base editing in human cells.Nature communications · 2024Article
- Nanoparticle-mediated delivery of non-viral gene editing technology to the brain.Progress in neurobiology · 2024Review
- Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells.Nature communications · 2023Article
- Raising Climate-Resilient Crops: Journey From the Conventional Breeding to New Breeding Approaches.Current genomics · 2021Review
- Points of View on the Tools for Genome/Gene Editing.International journal of molecular sciences · 2021Review
- Genome engineering and disease modelingWorld journal of stem cells · 2021Review
- Gene Therapy for Cardiovascular Disease: Basic Research and Clinical Prospects.Frontiers in cardiovascular medicine · 2021Review
- Expanding the editable genome and CRISPR-Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking.Nucleic acids research · 2020Article
- Target-specific gene delivery in plant systems and their expression: Insights into recent developments.Journal of biosciences · 2020Review
- Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks.Nature communications · 2019Article
- CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations.Nature communications · 2019Article
- Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns.APL bioengineering · 2018Article
- Review
- Initiation of homologous recombination at DNA nicks.Nucleic acids research · 2018Review
- New breeding technique "genome editing" for crop improvement: applications, potentials and challenges.3 Biotech · 2018Review
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
28 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
Abstract
Engineered zinc finger nucleases (ZFNs) induce DNA double-strand breaks at specific recognition sequences and can promote efficient introduction of desired insertions, deletions or substitutions at or near the cut site via homology-directed repair (HDR) with a double- and/or single-stranded donor DNA template. However, mutagenic events caused by error-prone non-homologous end-joining (NHEJ)-mediated repair are introduced with equal or higher frequency at the nuclease cleavage site. Furthermore, unintended mutations can also result from NHEJ-mediated repair of off-target nuclease cleavage sites. Here, we describe a simple and general method for converting engineered ZFNs into zinc finger nickases (ZFNickases) by inactivating the catalytic activity of one monomer in a ZFN dimer. ZFNickases show robust strand-specific nicking activity in vitro. In addition, we demonstrate that ZFNickases can stimulate HDR at their nicking site in human cells, albeit at a lower frequency than by the ZFNs from which they were derived. Finally, we find that ZFNickases appear to induce greatly reduced levels of mutagenic NHEJ at their target nicking site. ZFNickases thus provide a promising means for inducing HDR-mediated gene modifications while reducing unwanted mutagenesis caused by error-prone NHEJ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.