ArticleBiotechnology for biofuels2012
Optimization of a synthetic mixture composed of major Trichoderma reesei enzymes for the hydrolysis of steam-exploded wheat straw.
Article in Biotechnology for biofuels, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Robust Production of Cellulases to Develop a Low-Cost Sugar Platform.Advances in biochemical engineering/biotechnology · 2026Review
- Screening, Characterization and Comparison of Endoglucanases/Xylanases from Thermophilic Fungi: AInternational journal of molecular sciences · 2025Article
- Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh.Scientific reports · 2024Article
- Efficient Constitutive Expression of Cellulolytic Enzymes inJournal of microbiology and biotechnology · 2021Article
- Synergistic Action of a Lytic Polysaccharide Monooxygenase and a Cellobiohydrolase fromApplied and environmental microbiology · 2020Article
- Comparison of Calcium Oxide and Calcium Peroxide Pretreatments of Wheat Straw for Improving Biohydrogen Production.ACS omega · 2020Article
- Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.Microbial cell factories · 2019Article
- Enzymatic hydrolysis of cellulosic materials using synthetic mixtures of purified cellulases bioengineered at3 Biotech · 2018Article
- Robust Surface Plasmon Resonance Chips for Repetitive and Accurate Analysis of Lignin-Peptide Interactions.ACS omega · 2018Article
- One-step process of hydrothermal and alkaline treatment of wheat straw for improving the enzymatic saccharification.Biotechnology for biofuels · 2018Article
- Fine-Tuned Enzymatic Hydrolysis of Organosolv Pretreated Forest Materials for the Efficient Production of Cellobiose.Frontiers in chemistry · 2018Article
- Production of highly efficient cellulase mixtures by genetically exploiting the potentials of Trichoderma reesei endogenous cellulases for hydrolysis of corncob residues.Microbial cell factories · 2017Article
- Expressing accessory proteins in cellulolyticBiotechnology for biofuels · 2017Article
- Type-dependent action modes ofBiotechnology for biofuels · 2017Article
- Conferring cellulose-degrading ability toBiotechnology for biofuels · 2017Article
- Development of Thermophilic Tailor-Made Enzyme Mixtures for the Bioconversion of Agricultural and Forest Residues.Frontiers in microbiology · 2016Article
- Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid-substrate fermentation.Biotechnology for biofuels · 2016Article
- Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.BioMed research international · 2015Article
- Enzymatic cellulose hydrolysis: enzyme reusability and visualization of β-glucosidase immobilized in calcium alginate.Molecules (Basel, Switzerland) · 2014Article
- Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass.Biotechnology for biofuels · 2014Article
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5 authors.
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Abstract
backgroundAn efficient hydrolysis of lignocellulosic substrates to soluble sugars for biofuel production necessitates the interplay and synergistic interaction of multiple enzymes. An optimized enzyme mixture is crucial for reduced cost of the enzymatic hydrolysis step in a bioethanol production process and its composition will depend on the substrate and type of pretreatment used. In the present study, an experimental design was used to determine the optimal composition of a Trichoderma reesei enzyme mixture, comprising the main cellulase and hemicellulase activities, for the hydrolysis of steam-exploded wheat straw.
methodsSix enzymes, CBH1 (Cel7a), CBH2 (Cel6a), EG1 (Cel7b), EG2 (Cel5a), as well as the xyloglucanase Cel74a and the xylanase XYN1 (Xyl11a) were purified from a T. reesei culture under lactose/xylose-induced conditions. Sugar release was followed in milliliter-scale hydrolysis assays for 48 hours and the influence of the mixture on initial conversion rates and final yields is assessed.
resultsThe developed model could show that both responses were strongly correlated. Model predictions suggest that optimal hydrolysis yields can be obtained over a wide range of CBH1 to CBH2 ratios, but necessitates a high proportion of EG1 (13% to 25%) which cannot be replaced by EG2. Whereas 5% to 10% of the latter enzyme and a xylanase content above 6% are required for highest yields, these enzymes are predicted to be less important in the initial stage of hydrolysis.
conclusionsThe developed model could reliably predict hydrolysis yields of enzyme mixtures in the studied domain and highlighted the importance of the respective enzyme components in both the initial and the final hydrolysis phase of steam-exploded wheat straw.
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