ArticleCellular reprogramming2012
Valproic acid improves the in vitro development competence of bovine somatic cell nuclear transfer embryos.
Article in Cellular reprogramming, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Trichostatin A-Induced Epigenetic Modifications and Their Influence on the Development of Porcine Cloned Embryos Derived from Bone Marrow-Mesenchymal Stem Cells.International journal of molecular sciences · 2025Article
- Article
- VPA selectively regulates pluripotency gene expression on donor cell and improve SCNT embryo development.In vitro cellular & developmental biology. Animal · 2018Article
- Valproic Acid Exposure of Pregnant Rats During Organogenesis Disturbs Pancreas Development in Insulin Synthesis and Secretion of the Offspring.Toxicological research · 2018Article
- Factors and molecules that could impact cell differentiation in the embryo generated by nuclear transfer.Organogenesis · 2017Review
- Melatonin enhances the developmental competence of porcine somatic cell nuclear transfer embryos by preventing DNA damage induced by oxidative stress.Scientific reports · 2017Article
- Ascorbic acid increases demethylation in somatic cell nuclear transfer embryos of the pig (Asian-Australasian journal of animal sciences · 2017Article
- Article
- Psammaplin a improves development and quality of somatic cell nuclear transfer mouse embryos.Cellular reprogramming · 2014Article
- The effect of valproic acid on bovine oocyte maturation and early embryonic development in vitro.Cytotechnology · 2014Article
- Development to term of cloned cattle derived from donor cells treated with valproic acid.PloS one · 2014Article
- Update on the state of play of Animal Health and Welfare and Environmental Impact of Animals derived from SCNT Cloning and their Offspring, and Food Safety of Products Obtained from those Animals.EFSA journal. European Food Safety Authority · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study was carried out to examine the effect of valproic acid (VPA), an important histone deacetylase inhibitor, on the in vitro development and expression of the epigenetic marker histone H3 lysine 9 (H3K9ac) in bovine somatic cell nuclear transfer (SCNT) embryos. We found that treatment with 4 mM VPA for 24 h could significantly improve the development of bovine SCNT embryos. Compared with the no-treatment group, the cleavage rate was higher (69.79 ± 0.99% vs. 65.11 ± 1.02%, p<0.05), as was the blastocyst rate (39.99 ± 1.29% vs. 34.87 ± 1.74%, p<0.05). Moreover, the rate of apoptosis (1.91 ± 0.48% vs. 5.67 ± 0.40%, p<0.05) in blastocysts was greatly reduced after VPA treatment. Valproic acid treatment also increased the immunofluorescent signal for H3K9ac in SCNT embryos in a pattern similar to that of in vitro fertilized (IVF) embryos. In conclusion, we demonstrated that VPA can significantly improve the in vitro developmental competence and enhance the nuclear reprogramming of bovine SCNT embryos.
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