ArticleProceedings of the National Academy of Sciences of the United States of America2012
Primary epimutations introduced during intracytoplasmic sperm injection (ICSI) are corrected by germline-specific epigenetic reprogramming.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Animal models accurately representing acute liver failure (Review).International journal of molecular medicine · 2026Review
- Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station.Stem cell reports · 2025Article
- Intracytoplasmic sperm injection induces transgenerational abnormalities in mice.The Journal of clinical investigation · 2023Article
- Promising therapeutic aspects in human genetic imprinting disorders.Clinical epigenetics · 2022Review
- Placental Abnormalities are Associated With Specific Windows of Embryo Culture in a Mouse Model.Frontiers in cell and developmental biology · 2022Article
- Programming of Embryonic Development.International journal of molecular sciences · 2021Review
- Epigenetic Mechanisms of ART-Related Imprinting Disorders: Lessons From iPSC and Mouse Models.Genes · 2021Review
- Hypomethylation of the DAZ3 promoter in idiopathic asthenospermia: a screening tool for liquid biopsy.Scientific reports · 2020Article
- Environmental Impact on Male (In)Fertility via Epigenetic Route.Journal of clinical medicine · 2020Review
- Assisted reproductive technologies induce temporally specific placental defects and the preeclampsia risk marker sFLT1 in mouse.Development (Cambridge, England) · 2020Article
- Loss of methylation of H19-imprinted gene derived from assisted reproductive technologies can be mitigated by cleavage-stage embryo transfer in mice.Journal of assisted reproduction and genetics · 2019Article
- The virtuous cycle of human genetics and mouse models in drug discovery.Nature reviews. Drug discovery · 2019Review
- Search for cis-acting factors and maternal effect variants in Silver-Russell patients with ICR1 hypomethylation and their mothers.European journal of human genetics : EJHG · 2019Article
- Multigenerational impacts of bile exposure are mediated by TGR5 signaling pathways.Scientific reports · 2018Article
- Identification of rare de novo epigenetic variations in congenital disorders.Nature communications · 2018Article
- Multifactorial analysis of the stochastic epigenetic variability in cord blood confirmed an impact of common behavioral and environmental factors but not of in vitro conception.Clinical epigenetics · 2018Article
- A Decade of Exploring the Mammalian Sperm Epigenome: Paternal Epigenetic and Transgenerational Inheritance.Frontiers in cell and developmental biology · 2018Review
- Global misregulation of genes largely uncoupled to DNA methylome epimutations characterizes a congenital overgrowth syndrome.Scientific reports · 2017Article
- Altered methylations of H19, Snrpn, Mest and Peg3 are reversible by developmental reprogramming in kidney tissue of ICSI-derived mice.Scientific reports · 2017Article
- Epigenetic inheritance of acquired traits through sperm RNAs and sperm RNA modifications.Nature reviews. Genetics · 2016Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The use of assisted reproductive technologies (ART) has become increasingly common worldwide and is now responsible for 2-3% of children born in developed countries. Multiple reports have suggested that ART-conceived children are more likely to develop rare epigenetic disorders such as Beckwith-Wiedemann Syndrome or Angelman Syndrome, both of which involve dysregulation of imprinted genes. Anecdotal reports suggest that animals produced with ART that manifest apparent epigenetic defects typically do not transmit these epimutations to subsequent generations when allowed to breed naturally, but this hypothesis has not been directly studied. We analyzed allele-specific DNA methylation and expression at three imprinted genes, H19, Snrpn, and Peg3, in somatic cells from adult mice generated with the use of intracytoplasmic sperm injection (ICSI), a type of ART. Epimutations were detected in most of the ICSI-derived mice, but not in somatic cells of their offspring produced by natural mating. We examined germ cells from the ICSI mice that exhibited epimutations in their somatic cells and confirmed normal epigenetic reprogramming of the three imprinted genes analyzed. Collectively, these results confirm that ART procedures can lead to the formation of primary epimutations, but while such epimutations are likely to be maintained indefinitely in somatic cells of the ART-derived individuals, they are normally corrected in the germ line by epigenetic reprogramming and thus, not propagated to subsequent generations.
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