ArticleProceedings of the National Academy of Sciences of the United States of America2012
Rapid creation of Arabidopsis doubled haploid lines for quantitative trait locus mapping.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Uncovering adaptation with a new Arabidopsis thaliana multiparent intercross population.Genetics · 2026Article
- Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Establishment and inheritance of minichromosomes from Arabidopsis haploid induction.Chromosoma · 2023Article
- Genetic Mapping of Genotype-by-Ploidy Effects inGenes · 2023Article
- A simple and highly efficient strategy to induce both paternal and maternal haploids through temperature manipulation.Nature plants · 2023Article
- QTL Analysis Reveals Conserved and Differential Genetic Regulation of Maize Lateral Angles above the Ear.Plants (Basel, Switzerland) · 2023Article
- Direct assessment of histone function using histone replacement.Trends in biochemical sciences · 2023Review
- Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus.BMC plant biology · 2021Article
- Species with Haploid or Doubled Haploid Protocols.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Recombination Pattern Characterization via Simulation Using Different Maize Populations.International journal of molecular sciences · 2020Article
- "Doubled-haploid" allohexaploid Brassica lines lose fertility and viability and accumulate genetic variation due to genomic instability.Chromosoma · 2019Article
- Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective.Journal of genetics · 2019Review
- Complex Patterns of Cannabinoid Alkyl Side-Chain Inheritance in Cannabis.Scientific reports · 2019Article
- Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans.Developmental cell · 2019Article
- Centromere histone H3- and phospholipase-mediated haploid induction in plants.Plant methods · 2019Review
- Quantitative trait loci for resistance to Flavobacterium psychrophilum in rainbow trout: effect of the mode of infection and evidence of epistatic interactions.Genetics, selection, evolution : GSE · 2018Article
- Maternal doubled haploid production in interploidy hybridization between Brassica napus and Brassica allooctaploids.Planta · 2018Article
- Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Expressed Centromere Specific Histone 3 (Frontiers in plant science · 2017Article
- Accelerated Generation of Selfed Pure Line Plants for Gene Identification and Crop Breeding.Frontiers in plant science · 2017Review
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9 authors.
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Abstract
Quantitative trait loci (QTL) mapping is a powerful tool for investigating the genetic basis of natural variation. QTL can be mapped using a number of different population designs, but recombinant inbred lines (RILs) are among the most effective. Unfortunately, homozygous RIL populations are time consuming to construct, typically requiring at least six generations of selfing starting from a heterozygous F(1). Haploid plants produced from an F(1) combine the two parental genomes and have only one allele at every locus. Converting these sterile haploids into fertile diploids (termed "doubled haploids," DHs) produces immortal homozygous lines in only two steps. Here we describe a unique technique for rapidly creating recombinant doubled haploid populations in Arabidopsis thaliana: centromere-mediated genome elimination. We generated a population of 238 doubled haploid lines that combine two parental genomes and genotyped them by reduced representation Illumina sequencing. The recombination rate and parental allele frequencies in our population are similar to those found in existing RIL sets. We phenotyped this population for traits related to flowering time and for petiole length and successfully mapped QTL controlling each trait. Our work demonstrates that doubled haploid populations offer a rapid, easy alternative to RILs for Arabidopsis genetic analysis.
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