ArticleProceedings of the National Academy of Sciences of the United States of America2012
Multiple self-splicing introns in the 16S rRNA genes of giant sulfur bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
- Recurrent Modular Assembly Drives Independent Origins of Group II Introns Encoding LAGLIDADG Homing Endonucleases.Genome biology and evolution · 2026Article
- Group I introns in tRNA genes of Patescibacteria.mSystems · 2026Article
- Comprehensive analysis of insertion sequences within rRNA genes of CPR bacteria and biochemical characterization of a homing endonuclease encoded by these sequences.Journal of bacteriology · 2024Article
- Complex bacterial diversity of Guaymas Basin hydrothermal sediments revealed by synthetic long-read sequencing (LoopSeq).Frontiers in microbiology · 2024Article
- Insight into the symbiotic lifestyle of DPANN archaea revealed by cultivation and genome analyses.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Distinct Expansion of Group II Introns During Evolution of Prokaryotes and Possible Factors Involved in Its Regulation.Frontiers in microbiology · 2022Article
- Plant-Microbe Interaction: Aboveground to Belowground, from the Good to the Bad.International journal of molecular sciences · 2021Review
- Comparative Analysis of 16S rRNA Gene and Metagenome Sequencing in Pediatric Gut Microbiomes.Frontiers in microbiology · 2021Article
- Giant sulfur bacteria (Beggiatoaceae) from sediments underlying the Benguela upwelling system host diverse microbiomes.PloS one · 2021Article
- Molecular diversity and profile analysis of virulence-associated genes in somePractical laboratory medicine · 2020Article
- Understanding and overcoming the pitfalls and biases of next-generation sequencing (NGS) methods for use in the routine clinical microbiological diagnostic laboratory.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- TA, GT and AC are significantly under-represented in open reading frames of prokaryotic and eukaryotic protein-coding genes.Molecular genetics and genomics : MGG · 2019Article
- Review
- A Bioinformatics Guide to Plant Microbiome Analysis.Frontiers in plant science · 2019Review
- Biosynthetic capacity, metabolic variety and unusual biology in the CPR and DPANN radiations.Nature reviews. Microbiology · 2018Review
- Article
- Article
- Community-like genome in single cells of the sulfur bacterium Achromatium oxaliferum.Nature communications · 2017Article
- Novel Large Sulfur Bacteria in the Metagenomes of Groundwater-Fed Chemosynthetic Microbial Mats in the Lake Huron Basin.Frontiers in microbiology · 2017Article
- Single sample resolution of rare microbial dark matter in a marine invertebrate metagenome.Scientific reports · 2016Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding the small subunit rRNA serves as a prominent tool for the phylogenetic analysis and classification of Bacteria and Archaea owing to its high degree of conservation and its fundamental function in living organisms. Here we show that the 16S rRNA genes of not-yet-cultivated large sulfur bacteria, among them the largest known bacterium Thiomargarita namibiensis, regularly contain numerous self-splicing introns of variable length. The 16S rRNA genes can thus be enlarged to up to 3.5 kb. Remarkably, introns have never been identified in bacterial 16S rRNA genes before, although they are the most frequently sequenced genes today. This may be caused in part by a bias during the PCR amplification step that discriminates against longer homologs, as we show experimentally. Such length heterogeneity of 16S rRNA genes has so far never been considered when constructing 16S rRNA-based clone libraries, even though an elongation of rRNA genes due to intervening sequences has been reported previously. The detection of elongated 16S rRNA genes has profound implications for common methods in molecular ecology and may cause systematic biases in several techniques. In this study, catalyzed reporter deposition-fluorescence in situ hybridization on both ribosomes and rRNA precursor molecules as well as in vitro splicing experiments were performed and confirmed self-splicing of the introns. Accordingly, the introns do not inhibit the formation of functional ribosomes.
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