ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Assessment of circadian rhythms in humans: comparison of real-time fibroblast reporter imaging with plasma melatonin.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 69 citations in OpenAlex.
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- Mathematical Analysis of Light-sensitivity Related Challenges in Assessment of the Intrinsic Period of the Human Circadian Pacemaker.Journal of biological rhythms · 2024Article
- Desynchronizing the sleep---wake cycle from circadian timing to assess their separate contributions to physiology and behaviour and to estimate intrinsic circadian period.Nature protocols · 2023Review
- Human circadian rhythm studies: Practical guidelines for inclusion/exclusion criteria and protocol.Neurobiology of sleep and circadian rhythms · 2022Review
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- Mistimed sleep and waking activity in humans disrupts glucocorticoid signalling transcripts and SP1, but not plasma cortisol rhythms.Frontiers in physiology · 2022Article
- Self-reported sleep quality is more closely associated with mental and physical health than chronotype and sleep duration in young adults: A multi-instrument analysis.Journal of sleep research · 2021Article
- Novel Approaches for Assessing Circadian Rhythmicity in Humans: A Review.Journal of biological rhythms · 2020Review
- Using Chronobiological Phenotypes to Address Heterogeneity in Bipolar Disorder.Molecular neuropsychiatry · 2020Review
- Idiopathic Hypersomnia Patients Revealed Longer Circadian Period Length in Peripheral Skin Fibroblasts.Frontiers in neurology · 2018Article
- Delayed sleep-wake phase disorder.Journal of thoracic disease · 2018Review
- Sex and ancestry determine the free-running circadian period.Journal of sleep research · 2017Article
- Daily Light Exposure Patterns Reveal Phase and Period of the Human Circadian Clock.Journal of biological rhythms · 2017Article
- Evaluation of circadian phenotypes utilizing fibroblasts from patients with circadian rhythm sleep disorders.Translational psychiatry · 2017Article
- Regulation of molecular clock oscillations and phagocytic activity via muscarinic CaScientific reports · 2017Article
- Article
- The Functional and Clinical Significance of the 24-Hour Rhythm of Circulating Glucocorticoids.Endocrine reviews · 2017Review
- Circadian Rhythm and Sleep Disruption: Causes, Metabolic Consequences, and Countermeasures.Endocrine reviews · 2016Review
- Molecular insights into chronotype and time-of-day effects on decision-making.Scientific reports · 2016Article
- Sex differences in the circadian regulation of sleep and waking cognition in humans.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
We compared the period of the rhythm of plasma melatonin, driven by the hypothalamic circadian pacemaker, to in vitro periodicity in cultured peripheral fibroblasts to assess the effects on these rhythms of a polymorphism of PER3 (rs57875989), which is associated with sleep timing. In vitro circadian period was determined using luminometry of cultured fibroblasts, in which the expression of firefly luciferase was driven by the promoter of the circadian gene Arntl (Bmal1). The period of the melatonin rhythm was assessed in a 9-d forced desynchrony protocol, minimizing confounding effects of sleep-wake and light-dark cycles on circadian rhythmicity. In vitro periods (32 participants, 24.61±0.33 h, mean±SD) were longer than in vivo periods (31 participants, 24.16±0.17 h; P<0.0001) but did not differ between PER3 genotypes (P>0.4). Analyses of replicate in vitro assessments demonstrated that circadian period was reproducible within individuals (intraclass correlation=0.62), but in vivo and in vitro period assessments did not correlate (P>0.9). In accordance with circadian entrainment theory, in vivo period correlated with the timing of melatonin (P<0.05) at baseline and with diurnal preference (P<0.05). Individual circadian rhythms can be reliably assessed in fibroblasts but may not correlate with physiological rhythms driven by the central circadian pacemaker.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.