ArticlePlant physiology2012
The phosphatidylcholine diacylglycerol cholinephosphotransferase is required for efficient hydroxy fatty acid accumulation in transgenic Arabidopsis.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 2 of them syntheses that pooled it.
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Who cites it
71 citing papers in PubMed, 2 syntheses or guidelines pooled it, 134 citations in OpenAlex.
- Conserved mechanisms of plant lipidome remodeling under heat and cold stresses revealed through a systematic review and meta-analysis.Journal of experimental botany · 2026Pooled it
- A toolkit for plant lipid engineering: Surveying the efficacies of lipogenic factors for accumulating specialty lipids.Frontiers in plant science · 2022Pooled it
- Lysophosphatidylcholine homeostasis via the Lands cycle regulates root growth in Arabidopsis.Plant & cell physiology · 2026Article
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Article
- Decreases in polyunsaturated fatty acid content improve heat stress tolerance during flowering and silicle development in pennycress (Frontiers in plant science · 2026Article
- Chromosome-level assembly and analysis of three hydroxy fatty acid-producing Physaria species.Nature communications · 2025Article
- Discovery of Hub Genes Involved in Seed Development and Lipid Biosynthesis in Sea Buckthorn (Plants (Basel, Switzerland) · 2025Article
- Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp.Microbial cell factories · 2025Article
- Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism.Plant physiology · 2025Review
- Review
- Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils.Nature communications · 2024Article
- 13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri.Journal of experimental botany · 2024Article
- Comprehensive identification and functional characterization of GhpPLA gene family in reproductive organ development.BMC plant biology · 2023Article
- Phosphatidylcholine:diacylglycerol cholinephosphotransferase's unique regulation of castor bean oil quality.Plant physiology · 2022Article
- Enhanced production of hydroxy fatty acids in Arabidopsis seed through modification of multiple gene expression.Biotechnology for biofuels and bioproducts · 2022Article
- Molecular and Physiological Responses ofInternational journal of molecular sciences · 2022Article
- Critical metabolic pathways and genes cooperate for epoxy fatty acid-enriched oil production in developing seeds of Vernonia galamensis, an industrial oleaginous plant.Biotechnology for biofuels and bioproducts · 2022Article
- Effective Mechanisms for Improving Seed Oil Production in Pennycress (Frontiers in plant science · 2022Article
- A Review of Erucic Acid Production in Brassicaceae Oilseeds: Progress and Prospects for the Genetic Engineering of High and Low-Erucic Acid Rapeseeds (Frontiers in plant science · 2022Review
11 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously identified an enzyme, phosphatidylcholine diacylglycerol cholinephosphotransferase (PDCT), that plays an important role in directing fatty acyl fluxes during triacylglycerol (TAG) biosynthesis. The PDCT mediates a symmetrical interconversion between phosphatidylcholine (PC) and diacylglycerol (DAG), thus enriching PC-modified fatty acids in the DAG pool prior to forming TAG. We show here that PDCT is required for the efficient metabolism of engineered hydroxy fatty acids in Arabidopsis (Arabidopsis thaliana) seeds. When a fatty acid hydroxylase (FAH12) from castor (Ricinus communis) was expressed in Arabidopsis seeds, the PDCT-deficient mutant accumulated only about half the amount of hydroxy fatty acids compared with that in the wild-type seeds. We also isolated a PDCT from castor encoded by the RcROD1 (Reduced Oleate Desaturation1) gene. Seed-specific coexpression of this enzyme significantly increased hydroxy fatty acid accumulation in wild type-FAH12 and in a previously produced transgenic Arabidopsis line coexpressing a castor diacylglycerol acyltransferase 2. Analyzing the TAG molecular species and regiochemistry, along with analysis of fatty acid composition in TAG and PC during seed development, indicate that PDCT acts in planta to enhance the fluxes of fatty acids through PC and enrich the hydroxy fatty acids in DAG, and thus in TAG. In addition, PDCT partially restores the oil content that is decreased in FAH12-expressing seeds. Our results add a new gene in the genetic toolbox for efficiently engineering unusual fatty acids in transgenic oilseeds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.