ArticleThe Journal of physiology2012
Purinergic neuromuscular transmission is absent in the colon of P2Y(1) knocked out mice.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 86 citations in OpenAlex.
- Pharmacological characterization of alpha adrenoceptor-mediated motor responses in the rat colon.Neurogastroenterology and motility · 2025Article
- Ca²⁺ signaling in myenteric interstitial cells of Cajal (ICC-MY) and their role as conditional pacemakers in the colon.Cell calcium · 2025Article
- Integrated responses of the SIP syncytium generate a major motility pattern in the colon.The Journal of physiology · 2024Article
- Modulation of intracellular calcium activity in interstitial cells of Cajal by inhibitory neural pathways within the internal anal sphincter.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- CaPhysiological reviews · 2024Review
- Smooth muscle cells, interstitial cells and neurons in the gallbladder (GB): Functional syncytium of electrical rhythmicity and GB motility (Review).International journal of molecular medicine · 2023Review
- Distribution and Function of Platelet-derived Growth Factor Receptor Alpha-positive Cells and Purinergic Neurotransmission in the Human Colon: Is It Different Between the Right and Left Colon?Journal of neurogastroenterology and motility · 2022Article
- Clinical application and research progress of extracellular slow wave recording in the gastrointestinal tract.World journal of gastrointestinal surgery · 2022Review
- CaAdvances in experimental medicine and biology · 2022Article
- Neurotransmitters responsible for purinergic motor neurotransmission and regulation of GI motility.Autonomic neuroscience : basic & clinical · 2021Article
- Burnstock and the legacy of the inhibitory junction potential and P2Y1 receptors.Purinergic signalling · 2021Review
- Extracellular metabolism of the enteric inhibitory neurotransmitter β-nicotinamide adenine dinucleotide (β-NAD) in the murine colon.The Journal of physiology · 2020Article
- NTPDase1 and -2 are expressed by distinct cellular compartments in the mouse colon and differentially impact colonic physiology and function after DSS colitis.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Tonic inhibition of murine proximal colon is due to nitrergic suppression of CaScientific reports · 2019Article
- Inhibitory Neural Regulation of the CaFrontiers in physiology · 2018Article
- P2Y1R is involved in visceral hypersensitivity in rats with experimental irritable bowel syndrome.World journal of gastroenterology · 2017Article
- Expression of Ecto-nucleoside Triphosphate Diphosphohydrolases-2 and -3 in the Enteric Nervous System Affects Inflammation in Experimental Colitis and Crohn's Disease.Journal of Crohn's & colitis · 2017Article
- Purinergic signaling during intestinal inflammation.Journal of molecular medicine (Berlin, Germany) · 2017Review
- Convergence of inhibitory neural inputs regulate motor activity in the murine and monkey stomach.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Upregulation of L-type calcium channels in colonic inhibitory motoneurons of P/Q-type calcium channel-deficient mice.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
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6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purinergic and nitrergic co-transmission is the dominant mechanism responsible for neural-mediated smooth muscle relaxation in the gastrointestinal tract. The aim of the present paper was to test whether or not P2Y(1) receptors are involved in purinergic neurotransmission using P2Y(1)(−/−) knock-out mice. Tension and microelectrode recordings were performed on colonic strips. In wild type (WT) animals, electrical field stimulation (EFS) caused an inhibitory junction potential (IJP) that consisted of a fast IJP (MRS2500 sensitive, 1 μm) followed by a sustained IJP (N(ω)-nitro-L-arginine (L-NNA) sensitive, 1 mm). The fast component of the IJP was absent in P2Y(1)(−/−) mice whereas the sustained IJP (L-NNA sensitive) was recorded. In WT animals, EFS-induced inhibition of spontaneous motility was blocked by the consecutive addition of L-NNA and MRS2500. In P2Y(1)(−/−) mice, EFS responses were completely blocked by L-NNA. In WT and P2Y(1)(−/−) animals, L-NNA induced a smooth muscle depolarization but ‘spontaneous' IJP (MRS2500 sensitive) could be recorded in WT but not in P2Y(1)(−/−) animals. Finally, in WT animals, 1 μm MRS2365 caused a smooth muscle hyperpolarization that was blocked by 1 μm MRS2500. In contrast, 1 μm MRS2365 did not modify smooth muscle resting membrane potential in P2Y(1)(−/−) mice. β-Nicotinamide adenine dinucleotide (β-NAD, 1 mm) partially mimicked the effect of MRS2365. We conclude that P2Y(1) receptors mediate purinergic neurotransmission in the gastrointestinal tract and β-NAD partially fulfils the criteria to participate in rodent purinergic neurotransmission. The P2Y(1)(−/−) mouse is a useful animal model to study the selective loss of purinergic neurotransmission.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.