ArticleInfection and immunity2012
Vitamin B12-mediated restoration of defective anaerobic growth leads to reduced biofilm formation in Pseudomonas aeruginosa.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- The long 5' UTR of nrdAB modulates mRNA levels, stability, and virulence in Pseudomonas aeruginosa PAO1.Scientific reports · 2025Article
- Potential application of vitamins to combat biofilm-mediated drug resistance by pathogenic bacteria.Future microbiology · 2025Review
- Spatial transcriptomics identifies novel Pseudomonas aeruginosa virulence factors.Cell genomics · 2025Article
- The FinO/ProQ-like protein PA2582 impacts antimicrobial resistance inFrontiers in microbiology · 2024Article
- Detection of Known and Novel Small Proteins inAnalytical chemistry · 2023Article
- Review
- Antivirulence activities of retinoic acids againstFrontiers in microbiology · 2023Article
- Allopatric Plant Pathogen Population Divergence following Disease Emergence.Applied and environmental microbiology · 2021Article
- The ThiL enzyme is a valid antibacterial target essential for both thiamine biosynthesis and salvage pathways inThe Journal of biological chemistry · 2020Article
- Patterns of inter- and intrasubspecific homologous recombination inform eco-evolutionary dynamics of Xylella fastidiosa.The ISME journal · 2019Article
- A Cobalamin Activity-Based Probe Enables Microbial Cell Growth and Finds New Cobalamin-Protein Interactions across Domains.Applied and environmental microbiology · 2018Article
- Aerobic Vitamin BFrontiers in microbiology · 2018Article
- Negative Control of RpoS Synthesis by the sRNA ReaL inFrontiers in microbiology · 2018Article
- A single point mutation in class III ribonucleotide reductase promoter renders Pseudomonas aeruginosa PAO1 inefficient for anaerobic growth and infection.Scientific reports · 2017Article
- Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection.Infection and immunity · 2016Article
- Article
- Pseudomonas aeruginosa Exhibits Deficient Biofilm Formation in the Absence of Class II and III Ribonucleotide Reductases Due to Hindered Anaerobic Growth.Frontiers in microbiology · 2016Article
- A novel siderophore system is essential for the growth of Pseudomonas aeruginosa in airway mucus.Scientific reports · 2015Article
- Phylogenetic analysis of vitamin B12-related metabolism in Mycobacterium tuberculosis.Frontiers in molecular biosciences · 2015Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa undergoes cell elongation and forms robust biofilms during anaerobic respiratory growth using nitrate (NO(3)(-)) as an alternative electron acceptor. Understanding the mechanism of cell shape change induced upon anaerobiosis is crucial to the development of effective treatments against P. aeruginosa biofilm infection. Here, we uncovered the molecular basis of anaerobiosis-triggered cell elongation and identified vitamin B(12) to be a molecule that can reinstate defective anaerobic growth of P. aeruginosa. The ratio of total cellular DNA content to protein content was significantly decreased in the PAO1 strain grown under anaerobic conditions, indicating that DNA replication is impaired during anaerobic growth. Anaerobic growth of PAO1 reached a higher cell density in the presence of vitamin B(12), an essential coenzyme of class II ribonucleotide reductase. In addition, cell morphology returned to a normal rod shape and transcription of stress-response genes was downregulated under the same anaerobic growth conditions. These results suggest that vitamin B(12), the production of which was suppressed during anaerobic growth, can restore cellular machineries for DNA replication and therefore facilitate better anaerobic growth of P. aeruginosa with normal cell division. Importantly, biofilm formation was substantially decreased when grown with vitamin B(12), further demonstrating that anaerobiosis-induced cell elongation is responsible for robust biofilm formation. Taken together, our data reveal mechanistic details of a morphological change that naturally occurs during anaerobic growth of P. aeruginosa and illustrates the ability of vitamin B(12) to modulate the biofilm-forming capacity of P. aeruginosa under such condition.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.