ArticleCurrent microbiology2012
A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina.
Article in Current microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 20 citations in OpenAlex.
- Genetic Transformation of the Marine Oleaginous Microalga, Marinichlorella sp. NKG400014.Marine biotechnology (New York, N.Y.) · 2025Article
- A Novel SmallCells · 2023Article
- Stable Recombinant-Gene Expression from aApplied and environmental microbiology · 2021Article
- Advantages of Heterotrophic Microalgae as a Host for Phytochemicals Production.Frontiers in bioengineering and biotechnology · 2021Review
- The Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Is Critical for Abiotic Stress Response in Wheat.International journal of molecular sciences · 2019Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins.World journal of microbiology & biotechnology · 2014Review
- Manipulating natural product biosynthetic pathways via DNA assembler.Current protocols in chemical biology · 2014Article
- Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold.ACS synthetic biology · 2013Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge for efficient transgene expression in Dunaliella salina is to find strong endogenous promoters to drive the transgene expression. In the present study, a novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter was cloned and used to drive expressions of the bialaphos resistance (bar) gene and of the N-terminal fragment of human canstatin (Can-N). The results showed that the bar gene was transcribed by the GAPDH promoter and integrated into the genome of the transformants of D. salina. Furthermore, the PCR identification, Southern and western blots indicated that Can-N was expressed in transgenic D. salina, demonstrating that the promoter of the D. salina GAPDH gene is suitable for driving expression of heterologous genes in transgenic D. salina.
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