ArticleHaematologica2012
Arachidonic acid depletion extends survival of cold-stored platelets by interfering with the [glycoprotein Ibα--14-3-3ζ] association.
Article in Haematologica, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- The role of platelet desialylation as a biomarker in primary immune thrombocytopenia: mechanisms and therapeutic perspectives.Frontiers in immunology · 2024Review
- Cold-stored platelets for acute bleeding in cardiac surgical patients: a narrative review.Canadian journal of anaesthesia = Journal canadien d'anesthesie · 2023Review
- Reduced platelet glycoprotein Ibα shedding accelerates thrombopoiesis and COX-1 recovery: implications for aspirin dosing regimen.Haematologica · 2023Article
- Platelets from 13-lined ground squirrels are resistant to cold storage lesions.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- The GPIb-IX complex on platelets: insight into its novel physiological functions affecting immune surveillance, hepatic thrombopoietin generation, platelet clearance and its relevance for cancer development and metastasis.Experimental hematology & oncology · 2022Review
- Immune Thrombocytopenic Purpura as a Hemorrhagic Versus Thrombotic Disease: An Updated Insight into Pathophysiological Mechanisms.Medicina (Kaunas, Lithuania) · 2022Review
- Reversible thrombocytopenia during hibernation originates from storage and release of platelets in liver sinusoids.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Article
- Primary Immune Thrombocytopenia: Novel Insights into Pathophysiology and Disease Management.Journal of clinical medicine · 2021Review
- Structure-function of platelet glycoprotein Ib-IX.Journal of thrombosis and haemostasis : JTH · 2020Review
- The role of neuraminidase 1 and 2 in glycoprotein Ibα-mediated integrin αIIbβ3 activation.Haematologica · 2020Article
- Not all (N)SAID and done: Effects of nonsteroidal anti-inflammatory drugs and paracetamol intake on platelets.Research and practice in thrombosis and haemostasis · 2020Review
- Article
- Towards increasing shelf life and haemostatic potency of stored platelet concentrates.Current opinion in hematology · 2018Review
- Review
- Protein kinase A determines platelet life span and survival by regulating apoptosis.The Journal of clinical investigation · 2017Article
- Dual role of the p38 MAPK/cPLA2 pathway in the regulation of platelet apoptosis induced by ABT-737 and strong platelet agonists.Cell death & disease · 2013Article
- Platelet interaction with von Willebrand factor is enhanced by shear-induced clustering of glycoprotein Ibα.Haematologica · 2013Article
- Improved platelet survival after cold storage by prevention of glycoprotein Ibα clustering in lipid rafts.Haematologica · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCold storage of platelets reduces bacterial growth and preserves their hemostatic properties better than current procedures do. However, storage at 0°C induces [14-3-3ζ-glycoprotein Ibα] association, 14-3-3ζ release from phospho-Bad, Bad activation and apoptosis. DESIGN AND
methodsWe investigated whether arachidonic acid, which also binds 14-3-3ζ, contributes to coldinduced apoptosis.
resultsCold storage activated P38-mitogen-activated protein kinase and released arachidonic acid, which accumulated due to cold inactivation of cyclooxygenase-1/thromboxane synthase. Accumulated arachidonic acid released 14-3-3ζ from phospho-Bad and decreased the mitochondrial membrane potential, which are steps in the induction of apoptosis. Addition of arachidonic acid did the same and its depletion made platelets resistant to cold-induced apoptosis. Incubation with biotin-arachidonic acid revealed formation of an [arachidonic acid-14-3-3ζ-glycoprotein Ibα] complex. Indomethacin promoted complex formation by accumulating arachidonic acid and released 14-3-3ζ from cyclo-oxygenase-1. Arachidonic acid depletion prevented the cold-induced reduction of platelet survival in mice.
conclusionsWe conclude that cold storage induced apoptosis through an [arachidonic acid-14-3-3ζ-glycoprotein Ibα] complex, which released 14-3-3ζ from Bad in an arachidonic acid-dependent manner. Although arachidonic acid depletion reduced agonist-induced thromboxane A(2) formation and aggregation, arachidonic acid repletion restored these functions, opening ways to reduce apoptosis during storage without compromising hemostatic functions post-transfusion.
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