ReviewSensors (Basel, Switzerland)2012
The evolution of the bacterial luciferase gene cassette (lux) as a real-time bioreporter.
Review in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 82 citations in OpenAlex.
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- Tumor-targeting bioluminescent bacteria forEngineering microbiology · 2025Article
- Light-Activated Gene Expression System Using a Caging-Group-Free Photoactivatable Dye.Angewandte Chemie (International ed. in English) · 2025Article
- Phagocytosis by macrophages decreases the radiance of bioluminescent Staphylococcus aureus.BMC microbiology · 2025Article
- Bioluminescent Systems for Theranostic Applications.International journal of molecular sciences · 2024Review
- Whole-Cell Biosensor for Iron Monitoring as a Potential Tool for Safeguarding Biodiversity in Polar Marine Environments.Marine drugs · 2024Review
- Cloning and expression of Burkholderia polyyne biosynthetic gene clusters in Paraburkholderia hosts provides a strategy for biopesticide development.Microbial biotechnology · 2022Article
- Development of Whole-Cell Biosensors for Screening of Peptidoglycan-Targeting Antibiotics in a Gram-Negative Bacterium.Applied and environmental microbiology · 2022Article
- Structure-Function Relationships in Temperature Effects on Bacterial Luciferases: Nothing Is Perfect.International journal of molecular sciences · 2022Article
- Bacterial bioluminescence assay for bioanalysis and bioimaging.Analytical and bioanalytical chemistry · 2022Review
- Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems.Frontiers in chemistry · 2022Review
- Article
- Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit.Frontiers in bioengineering and biotechnology · 2021Article
- Imaging of bioluminescentThe Journal of international medical research · 2020Article
- Estimation of Hg(II) in Soil Samples by Bioluminescent Bacterial BioreporterSensors (Basel, Switzerland) · 2020Article
- A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice byRSC advances · 2020Article
- Continuous and Real-Time In Vivo Autobioluminescent Imaging in a Mouse Model.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection.Applied and environmental microbiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is poised to become one of the first fully implantable detection systems for intra-organismal optical detection through direct marriage to an implantable photon-detecting digital chip. This review presents the basic biochemical background that allows the lux system to continuously autobioluminesce and highlights the important milestones in the use of lux-based bioreporters as they have evolved from chemical detection platforms in prokaryotic bacteria to rodent-based tumorigenesis study targets. In addition, the future of lux imaging using integrated circuit microluminometry to image directly within a living host in real-time will be introduced and its role in the development of dose/response therapeutic systems will be highlighted.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.