ArticleSensors (Basel, Switzerland)2012
Single-cell chemical lysis on microfluidic chips with arrays of microwells.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Integrated microfluidic device for DNA extraction using electric valves.Scientific reports · 2026Article
- Surface-Imprinted Polymer Coupled with Diffraction Gratings for Low-Cost, Label-Free and DifferentialBiosensors · 2026Article
- Recent Developments in Single-Cell Metabolomics by Mass Spectrometry─A Perspective.Journal of proteome research · 2025Review
- Spatiotemporal dissection of tumor microenvironment via in situ sensing and monitoring in tumor-on-a-chip.Biosensors & bioelectronics · 2023Article
- Microfluidics applications for high-throughput single cell sequencing.Journal of nanobiotechnology · 2021Review
- Review of Microfluidic Methods for Cellular Lysis.Micromachines · 2021Review
- The Development of Single-Cell Metabolism and Its Role in Studying Cancer Emergent Properties.Frontiers in oncology · 2021Review
- β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1).International journal of general medicine · 2021Article
- Rapid fabrication of sieved microwells and cross-flow microparticle trapping.Scientific reports · 2020Article
- Fully Automated Lab-On-A-Disc Platform for Loop-Mediated Isothermal Amplification Using Micro-Carbon-Activated Cell Lysis.Sensors (Basel, Switzerland) · 2020Article
- Microfluidic systems for hydrodynamic trapping of cells and clusters.Biomicrofluidics · 2020Review
- Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization.Sensors (Basel, Switzerland) · 2018Review
- Microfluidic-Based Single-Cell Study: Current Status and Future Perspective.Molecules (Basel, Switzerland) · 2018Review
- Lab-on-a-Disc Platform for Automated Chemical Cell Lysis.Sensors (Basel, Switzerland) · 2018Article
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- Cell Microarray Technologies for High-Throughput Cell-Based Biosensors.Sensors (Basel, Switzerland) · 2017Review
- Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles.Micromachines · 2017Article
- Microfluidic Devices for Forensic DNA Analysis: A Review.Biosensors · 2016Review
- Digital Microfluidics for Manipulation and Analysis of a Single Cell.International journal of molecular sciences · 2015Review
- T Cell Dynamic Activation and Functional Analysis in Nanoliter Droplet Microarray.Journal of clinical & cellular immunology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many conventional biochemical assays are performed using populations of cells to determine their quantitative biomolecular profiles. However, population averages do not reflect actual physiological processes in individual cells, which occur either on short time scales or nonsynchronously. Therefore, accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. Microfluidic chips with arrays of microwells were developed for single-cell chemical lysis in the present study. The cellular occupancy in 30-μm-diameter microwells (91.45%) was higher than that in 20-μm-diameter microwells (83.19%) at an injection flow rate of 2.8 μL/min. However, most of the occupied 20-μm-diameter microwells contained individual cells. The results of chemical lysis experiments at the single-cell level indicate that cell membranes were gradually lysed as the lysis buffer was injected; they were fully lysed after 12 s. Single-cell chemical lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.